Recombinant Human IL28B protein(Met1-Val196), His-tagged

• Cat.No.: IL28B-379H
• Product Overview: Recombinant Human IL28B (NP_742151.2) (Met 1-Val 196) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Human
• Tag: His
• Protein length: Met1-Val196
• Form: Lyophilized from sterile PBS, 500mM NaCl, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 4-16 ng/mL.
• Molecular Mass: The recombinant human IL28B consists of 186 amino acids and predictes a molecular mass of 21 kDa. In SDS-PAGE under reducing conditions, rhIL28B migrates as an approximately 23 kDa band.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 96 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: IL28B interleukin 28B (interferon, lambda 3) [ Homo sapiens ]
• Official Symbol: IL28B
• Synonyms: IL28B; interleukin 28B (interferon, lambda 3); interleukin 28B; interleukin-28B; IFNL3; IL 28B; IL28C; IL-28C; IFN-lambda-3; IFN-lambda-4; interleukin 28C; interleukin-28C; cytokine Zcyto22; interferon lambda-3; interferon lambda-4; interferon, lambda 3; interferon, lambda 4; IL-28B
• Gene ID: 282617
• mRNA Refseq: NM_172139
• Protein Refseq: NP_742151
• MIM: 607402
• UniProt ID: Q8IZI9

Reviews

07/24/2022

Activity retention time is very long and can be maintained for a long time.

02/04/2022

High cost performance and low preparation cost.

01/24/2022

It has a wide range of applications and can be used in various biological experiments.

Q&As

IL28B has made a breakthrough in treating what diseases?

IL28B has achieved remarkable results in the treatment of hepatitis C. The study found that patients with a specific genotype, which is related to IL28B, were better able to benefit from treatment with the hepatitis drug interferon. Therefore, the combination of IL28B with other drugs can improve the efficacy.

What is the mechanism of action of IL28B?

IL28B can regulate the immune response of cells and promote the occurrence of insulin-dependent cytotoxicity mediated by NK cells, thus playing an antiviral role.

IL28B purification ofs have?

The purification methods of IL28B include ion exchange, gel filtration, affinity chromatography, countercurrent chromatography, etc.

How many therapeutic doses of IL28B are usually used?

The dose of treatment with IL28B varies depending on the patient. When treating hepatitis C, the dose is usually given once a week, 500ug or another dose.

What are the adverse reactions of IL28B?

The adverse reactions of IL28B include fever, headache, fatigue, muscle aches, nausea, diarrhea and other symptoms, and in severe cases, allergic reactions and liver damage.

Does IL28B affect the function of other organs in the body?

IL28B will have a certain impact on the function of other organs of the body, such as the liver, kidneys, etc., but the use of normal doses in clinical practice is generally safe.