Recombinant Human IL2RG, Gamma, Fc Chimera

• Cat.No.: IL2RG-119H
• Product Overview: Recombinant Human interleukin 2 receptor, gamma (severe combined immunodeficiency) protein was expressed in modifiedhuman 293 cellsencoding the signal peptide and extracellular domain of human Interleukin 2 receptor gamma chain (aa 1-259). Itwas fused to the Fc region of human IgG1 (aa 93-330).

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 1-259 a.a.
• Description: Interleukin-2 (IL-2) is a pleiotropic glycoprotein that mediates the production of regulatory T cells and promotes T cell dependent tolerance. Mutations in the gene encoding IL-2R gamma results in X-linked severe combined immunodeficiency, which is characterised by an absence of T and NK cells, and near normal numbers of functionally deficient B cells.
• Amino Acid Sequence: LNTTILTPN GNEDTTADFFLTT MPTDSL SVSTLPLP EVQCFVFNV EYMNCTWN SSSEPQPTN L T L H YWYK NSDNDK VQKCSH YLFSEEI TSGCQL QKKEIHL YQTFVV QLQDPR EP R RQ ATQ MLKL QNLVIPW APENLTL HKLSESQ LELNWN NRFLNHC LEHLVQY R TDWDHS WTEQS VDYRHK FSLPSV DGQKRY TFRVRS RFNPLC GSAQHW S EWSHP IHWGS NTSKE NPFLFA WIPKVD KKVEP KSCDK THTCPP CPAPE L LGGPS VFLFPP KPKDTL MISRTP EVTCVV VDVSHE DPEVKF NWYVDG VEVHN AKTKPR EEQYN STYRVV SVLTVL HQDWL NGKEY KCKVS NKALPAPIE KTISKA KGQP REPQVYT LPPSRDELTKN QVSLTCLVKGFY P SDIAVEWESN GQ PENNYKTTP PVLDSDG SFFLYSKL TVDKSRW QQGNVFS CSVMHEAL HNHY TQ K S LSLSPGK.
• Molecular Mass: IL-2R gamma-Fc Chimera migrates as a band between 75 and 105 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL-2R gamma-Fc that has a predicted molecular mass of 55.1 kDa.
• PI: IL-2R gamma-Fc Chimera separates into a number of isoforms with a pI between 4.9 and 7.2 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-2 R gamma-Fc that has a predicted pI of 6.68.
• % Carbohydrate: Purified IL-2R gamma-Fc Chimera consists of 25-50% carbohydrate by weight.
• Glycosylation: IL-2R gamma-Fc Chimera has N-linked and O-linked oligosaccharides.
• Purity: >95%, as determined by SDS-PAGE and visualized by silver stain.
• Formulation: When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose.
• Reconstitution: It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial.
• Storage: Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended.

Gene Information

• Gene Name: IL2RG interleukin 2 receptor, gamma (severe combined immunodeficiency) [ Homo sapiens ]
• Synonyms: IL2RG; interleukin 2 receptor, gamma (severe combined immunodeficiency); CD132; IMD4; SCIDX; SCIDX1; common cytokine receptor gamma chain; interleukin 2 receptor gamma chain; interleukin 2 receptor, gamma; p64; interleukin 2 receptor, gamma; Interleukin-2 receptor gamma chain; IL-2R gamma chain; CD132 antigen
• Gene ID: 3561
• mRNA Refseq: NM_000206
• Protein Refseq: NP_000197
• MIM: 308380
• UniProt ID: P31785
• Chromosome Location: Xq13.1
• Pathway: Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway; Endocytosis; Primary immunodeficiency e
• Function: interleukin-2 receptor activity; protein binding; receptor activity