| Cat. No. : |
IL2RG-119H |
| Description : |
Interleukin-2 (IL-2) is a pleiotropic glycoprotein that mediates the production of regulatory T cells and promotes T cell dependent tolerance. Mutations in the gene encoding IL-2R gamma results in X-linked severe combined immunodeficiency, which is characterised by an absence of T and NK cells, and near normal numbers of functionally deficient B cells. |
| Source : |
Human 293 cells. |
| Amino Acid Sequence : |
LNTTILTPN GNEDTTADFFLTT MPTDSL SVSTLPLP EVQCFVFNV EYMNCTWN SSSEPQPTN L T L H YWYK NSDNDK VQKCSH YLFSEEI TSGCQL QKKEIHL YQTFVV QLQDPR EP R RQ ATQ MLKL QNLVIPW APENLTL HKLSESQ LELNWN NRFLNHC LEHLVQY R TDWDHS WTEQS VDYRHK FSLPSV DGQKRY TFRVRS RFNPLC GSAQHW S EWSHP IHWGS NTSKE NPFLFA WIPKVD KKVEP KSCDK THTCPP CPAPE L LGGPS VFLFPP KPKDTL MISRTP EVTCVV VDVSHE DPEVKF NWYVDG VEVHN AKTKPR EEQYN STYRVV SVLTVL HQDWL NGKEY KCKVS NKALPAPIE KTISKA KGQP REPQVYT LPPSRDELTKN QVSLTCLVKGFY P SDIAVEWESN GQ PENNYKTTP PVLDSDG SFFLYSKL TVDKSRW QQGNVFS CSVMHEAL HNHY TQ K S LSLSPGK. |
| Molecular Mass : |
IL-2R gamma-Fc Chimera migrates as a band between 75 and 105 kDa in SDS-PAGE due to post-translation modifications, in particular glycosylation. This compares with the unmodified IL-2R gamma-Fc that has a predicted molecular mass of 55.1 kDa. |
| PI : |
IL-2R gamma-Fc Chimera separates into a number of isoforms with a pI between 4.9 and 7.2 in 2D PAGE due to post-translational modifications, in particular glycosylation. This compares with the unmodified IL-2 R gamma-Fc that has a predicted pI of 6.68. |
| % Carbohydrate : |
Purified IL-2R gamma-Fc Chimera consists of 25-50% carbohydrate by weight. |
| Glycosylation : |
IL-2R gamma-Fc Chimera has N-linked and O-linked oligosaccharides. |
| Purity : |
>95%, as determined by SDS-PAGE and visualized by silver stain. |
| Formulation : |
When reconstituted in 0.5 ml sterile phosphate-buffered saline, the solution will contain 1% human serum albumin (HSA) and 10% trehalose. |
| Reconstitution : |
It is recommended that 0.5 ml of sterile phosphate-buffered saline be added to the vial. |
| Storage : |
Lyophilized products should be stored at 2 to 8°C. Following reconstitution short-term storage at 4°C is recommended, and longer-term storage of aliquots at -18 to -20°C. Repeated freeze thawing is not recommended. |
