| Species : |
Human |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
20-152 aa |
| Form : |
Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| Bio-activity : |
Interleukin 3 is an interleukin, a type of biological signal that can improve the body's natural response to disease as part of the immune system. It acts by binding to the interleukin 3 receptor IL-3 synergizes with other cytokines to stimulate the growth of immature progenitor cells of all lineages, and is therefore a multi-lineage colony-stimulating factor (CSF). It prevents cell death and promotes the survival of macrophages, mast cells, and megakaryocytes. To test the effect of IL-3 on cell proliferation, TF-1 cells were seeded into triplicate wells of 96-well plates at a density of 8,000 cells/well with 2% serum standard 1640 which contains various concentrations of recombinant human IL-3. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 µl of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 2-4 hours at 37°C. Proliferation of TF-1 cells after incubation with IL-3 for72h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 (Cell Counting Kit-8 ) assay after incubation with recombinant human IL-3 for 72h. It was obvious that IL-3 significantly increased cell viability of TF-1 cells. The ED50 is 0.033-0.3 ng/ml. |
| Molecular Mass : |
16.7kDa |
| Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
| Reconstitution : |
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |
