Active Recombinant Human IL5RA protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: IL5RA-344HB
• Product Overview: Biotinylated Active Recombinant Human IL5RA protein(Q01344-1)(Asp21-Glu335) is expressed from HEK293 with His tag and Avi tag at the C-Terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Avi&His
• Protein Length: 21-335 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4).
• Bio-activity: Immobilized Human IL-5, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.15μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 38.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: IL5RA interleukin 5 receptor, alpha [ Homo sapiens ]
• Official Symbol: IL5RA
• Synonyms: IL5RA; interleukin 5 receptor, alpha; IL5R; interleukin-5 receptor subunit alpha; CD125; CDw125; CD125 antigen; IL-5R subunit alpha; IL-5 receptor subunit alpha; interleukin 5 receptor type 3; interleukin-5 receptor alpha chain; HSIL5R3; MGC26560
• Gene ID: 3568
• mRNA Refseq: NM_000564
• Protein Refseq: NP_000555
• MIM: 147851
• UniProt ID: Q01344