Recombinant Human IRAK4 Protein (E154-S460), Tag Free

• Cat.No.: IRAK4-1168H
• Product Overview: Recombinant Human IRAK4(E154-S460 end) Protein was expressed in Insect cell.

Specification

• Description: Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation to form the Myddosome together with IRAK2. Phosphorylates initially IRAK1, thus stimulating the kinase activity and intensive autophosphorylation of IRAK1. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates NCF1 and regulates NADPH oxidase activation after LPS stimulation suggesting a similar mechanism during microbial infections.
• Source: Insect cell
• Species: Human
• Tag: Non
• Form: Liquid
• Protein length: E154-S460
• Endotoxin: < 0.01 EU per μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade.
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50 mM Tris-HCl (pH7.5), 200 mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Gene Name: IRAK4 interleukin-1 receptor-associated kinase 4 [ Homo sapiens (human) ]
• Official Symbol: IRAK4
• Synonyms: IRAK4; interleukin-1 receptor-associated kinase 4; NY REN 64; renal carcinoma antigen NY-REN-64; IPD1; REN64; IRAK-4; NY-REN-64
• Gene ID: 51135
• mRNA Refseq: NM_001114182
• Protein Refseq: NP_001107654
• MIM: 606883
• UniProt ID: Q9NWZ3

Reviews

11/16/2021

Its potency and specificity make it a valuable tool for studying specific biological pathways or mechanisms.

03/01/2020

Their technical expertise and experience in the field allow them to provide invaluable guidance and assistance.

07/16/2018

They can offer insights on experimental design, optimal usage protocols, and troubleshoot any challenges that may arise during the course of the trial.

Q&As

What conditions are associated with IRAK4 dysregulation?

Rheumatoid arthritis, lupus, inflammatory bowel diseases, and certain cancers may involve IRAK4.

Are there any ongoing clinical trials involving IRAK4 inhibitors?

Yes, several trials are investigating IRAK4 inhibitors' effectiveness and safety in various diseases.

What are the methods used to target IRAK4 in treatment?

Small molecule inhibitors and monoclonal antibodies are primary methods for modulating IRAK4 activity.

How does the expression of IRAK4 differ in healthy individuals and those with autoimmune diseases?

Individuals with autoimmune diseases often show increased IRAK4 expression compared to healthy individuals.

How does IRAK4 inhibition impact the immune response?

By blocking IRAK4, the overactive immune response in certain diseases can be suppressed.