Recombinant Human ITGAV protein, His-tagged

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Recombinant Human ITGAV protein, His-tagged

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Cat.No. : ITGAV-6763H
Product Overview : Recombinant Human ITGAV aa. (Pro560~Trp744) fused with N-terminal His tag was produced in E. coli cells.
Description : The product of this gene belongs to the integrin alpha chain family. Integrins are heterodimeric integral membrane proteins composed of an alpha subunit and a beta subunit that function in cell surface adhesion and signaling. The encoded preproprotein is proteolytically processed to generate light and heavy chains that comprise the alpha V subunit. This subunit associates with beta 1, beta 3, beta 5, beta 6 and beta 8 subunits. The heterodimer consisting of alpha V and beta 3 subunits is also known as the vitronectin receptor. This integrin may regulate angiogenesis and cancer progression. Alternative splicing results in multiple transcript variants. Note that the integrin alpha 5 and integrin alpha V subunits are encoded by distinct genes.
Source : E. coli
Species : Human
Tag : His
Form : Freeze-dried powder
Molecular Mass : 24kDa as determined by SDS-PAGE reducing conditions.
Protein length : Pro560~Trp744
Endotoxin : <1.0EU per 1ug (determined by the LAL method)
Purity : >98%
Characteristic : The isoelectric point is 5.1.
Applications : SDS-PAGE; WB; ELISA; IP; CoIP; ReporterAssays; Purification; Amine Reactive Labeling.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200ug/mL
Storage buffer : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% Trehalose and Proclin300.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
SDS-PAGE :

Gene Name : ITGAV integrin subunit alpha V [ Homo sapiens (human) ]
Official Symbol : ITGAV
Synonyms : CD51; MSK8; VNRA; VTNR; antigen identified by monoclonal antibody L230; integrin alphaVbeta3; integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51); vitronectin receptor subunit alpha; integrin alpha-V
Gene ID : 3685
mRNA Refseq : NM_001144999.2
Protein Refseq : NP_001138471.1
UniProt ID : P06756
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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