Recombinant Human LEFTY2 protein(Phe78-Pro366), hFc-tagged

• Cat.No.: LEFTY2-799H
• Product Overview: Recombinant Human LeftyA (NP_003231.2) (Phe 78-Pro 366) was expressed in HEK293, fused with the Fc region of Human IgG1 at the N-terminus.

Specification

• Species: Human
• Source: HEK293
• Tag: Fc
• Protein Length: 78-366 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit recombinant human BMP4 induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 4-16 μg/mL in the presence of 50 ng/mL of recombinant human BMP4.
• Molecular Mass: The recombinant human LeftyA/Fc is a disulfide-linked homodimer The reduced monomer consists of 526 amino acids and has a predicted molecular mass of 58.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh LeftyA/Fc monomer is approximately 70 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: LEFTY2 left-right determination factor 2 [ Homo sapiens ]
• Official Symbol: LEFTY2
• Synonyms: LEFTY2; left-right determination factor 2; EBAF, endometrial bleeding associated factor (left right determination, factor A; transforming growth factor beta superfamily) , TGFB4; LEFTA; LEFTYA; transforming growth factor; beta 4 (endometrial bleeding associated factor; LEFTY A); TGF-beta-4; protein lefty-2; protein lefty-A; left-right determination factor A; transforming growth factor beta-4; endometrial bleeding-associated factor; EBAF; TGFB4; MGC46222
• Gene ID: 7044
• mRNA Refseq: NM_003240
• Protein Refseq: NP_003231
• UniProt ID: O00292