Recombinant Human MAP3K1

• Cat.No.: MAP3K1-30190TH
• Product Overview: Recombinant fragment of Human MEKK1 (amino acids 900-1748) with N-terminal proprietary tag, 155kDa.

Specification

• Description: The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins.
• Protein length: 849 amino acids
• Molecular Weight: 155.000kDa inclusive of tags
• Source: Baculovirus infected sf9 cells
• Biological activity: The Specific activity of MAP3K1-30190TH was determined to be 820 nmol/min/mg.
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 0.01% Triton-X-100, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Contains 1 protein kinase domain.Contains 1 RING-type zinc finger.Contains 1 SWIM-type zinc finger.
• Tag: Non

Gene Information

• Gene Name: MAP3K1 mitogen-activated protein kinase kinase kinase 1 [ Homo sapiens ]
• Official Symbol: MAP3K1
• Synonyms: MAP3K1; mitogen-activated protein kinase kinase kinase 1; MEKK1; MAPKKK1; MEKK
• Gene ID: 4214
• mRNA Refseq: NM_005921
• Protein Refseq: NP_005912
• MIM: 600982
• Uniprot ID: Q13233
• Chromosome Location: 5q11.2
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis Modulation by HSP70, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem; CD40/CD40L signaling, organism-specific biosystem
• Function: ATP binding; JUN kinase binding; JUN kinase kinase activity; JUN kinase kinase kinase activity; MAP kinase kinase kinase activity

Reviews

12/20/2020

The bands of proteins in Western Blotting experiments were very clear.

11/10/2020

The MAP3K1 protein is highly recommended for its exceptional performance in ELISA assays.

09/30/2017

Researchers can rely on the MAP3K1 protein to deliver precise results, enhancing the credibility and efficiency of their experiments.

Q&As

How is MAP3K1 protein expression measured in clinical settings?

MAP3K1 protein expression can be measured using immunohistochemistry (IHC) or Western blotting in clinical settings.

Are there any diseases associated with MAP3K1 protein mutations?

Yes, mutations in the MAP3K1 gene have been linked to several diseases, including breast cancer, lung cancer, and colon cancer.

What are the implications of MAP3K1 protein in drug resistance?

MAP3K1 protein has been implicated in the development of drug resistance in certain cancer types, highlighting its importance in personalized medicine and treatment response.

What are the potential diagnostic applications of MAP3K1 protein analysis?

MAP3K1 protein analysis can be used for diagnostic purposes to identify specific molecular subtypes of cancer and to predict disease prognosis.

How is MAP3K1 protein targeted in precision medicine approaches?

Precision medicine approaches may involve targeting MAP3K1 protein through specific inhibitors or monoclonal antibodies to inhibit its oncogenic activity.