Recombinant Human MAP3K1

• Cat.No.: MAP3K1-30190TH
• Product Overview: Recombinant fragment of Human MEKK1 (amino acids 900-1748) with N-terminal proprietary tag, 155kDa.

Specification

• Description: The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins.
• Protein length: 849 amino acids
• Molecular Weight: 155.000kDa inclusive of tags
• Source: Baculovirus infected sf9 cells
• Biological activity: The Specific activity of MAP3K1-30190TH was determined to be 820 nmol/min/mg.
• Form: Liquid
• Purity: by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 25% Glycerol, 0.01% Triton-X-100, 50mM Tris HCl, 150mM Sodium chloride, 10mM Glutathione, 0.25mM DTT, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
• Storage: Shipped on dry ice. Upon delivery aliquot and store at -80oC. Avoid freeze / thaw cycles.
• Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.Contains 1 protein kinase domain.Contains 1 RING-type zinc finger.Contains 1 SWIM-type zinc finger.

Gene Information

• Gene Name: MAP3K1 mitogen-activated protein kinase kinase kinase 1 [ Homo sapiens ]
• Official Symbol: MAP3K1
• Synonyms: MAP3K1; mitogen-activated protein kinase kinase kinase 1; MEKK1; MAPKKK1; MEKK
• Gene ID: 4214
• mRNA Refseq: NM_005921
• Protein Refseq: NP_005912
• MIM: 600982
• Uniprot ID: Q13233
• Chromosome Location: 5q11.2
• Pathway: Activated TLR4 signalling, organism-specific biosystem; Apoptosis, organism-specific biosystem; Apoptosis Modulation by HSP70, organism-specific biosystem; BCR signaling pathway, organism-specific biosystem; CD40/CD40L signaling, organism-specific biosystem
• Function: ATP binding; JUN kinase binding; JUN kinase kinase activity; JUN kinase kinase kinase activity; MAP kinase kinase kinase activity