Recombinant Human MAP3K1 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: MAP3K1-2121H
• Product Overview: MAP3K1 MS Standard C13 and N15-labeled recombinant protein (NP_005912) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: The protein encoded by this gene is a serine/threonine kinase and is part of some signal transduction cascades, including the ERK and JNK kinase pathways as well as the NF-kappa-B pathway. The encoded protein is activated by autophosphorylation and requires magnesium as a cofactor in phosphorylating other proteins. This protein has E3 ligase activity conferred by a plant homeodomain (PHD) in its N-terminus and phospho-kinase activity conferred by a kinase domain in its C-terminus.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 164.3 kDa
• AA Sequence: MAAAAGNRASSSGFPGARATSPEAG GGGGALKASSAPAAAAGLLREAGSG GRERADWRRRQLRKVRSVELDQLPE QPLFLAASPPASSTSPSPEPADAAG SGTGFQPVAVPPPHGAASRGGAHLT ESVAAPDSGASSPAAAEPGEKRAPA AEPSPAAAPAGREMENKETLKGLHK MDDRPEERMIREKLKATCMPAWKHE WLERRNRRGPVVVKPIPVKGDGSEM NHLAAESPGEVQASAASPASKGRRS PSPGNSPSGRTVKSESPGVRRKRVS PVPFQSGRITPPRRAPSPDGFSPYS PEETNRRVNKVMRARLYLLQQIGPN SFLIGGDSPDNKYRVFIGPQNCSCA RGTFCIHLLFVMLRVFQLEPSDPML WRKTLKNFEVESLFQKYHSRRSSRI KAPSRNTIQKFVSRMSNSHTLSSSS TSTSSSENSIKDEEEQMCPICLLGM LDEESLTVCEDGCRNKLHHHCMSIW AEECRRNREPLICPLCRSKWRSHDF YSHELSSPVDSPSSLRAAQQQTVQQ QPLAGSRRNQESNFNLTHYGTQQIP PAYKDLAEPWIQVFGMELVGCLFSR NWNVREMALRRLSHDVSGALLLANG ESTGNSGGSSGSSPSGGATSGSSQT SISGDVVEACCSVLSMVCADPVYKV YVAALKTLRAMLVYTPCHSLAERIK LQRLLQPVVDTILVKCADANSRTSQ LSISTLLELCKGQAGELAVGREILK AGSIGIGGVDYVLNCILGNQTESNN WQELLGRLCLIDRLLLEFPAEFYPH IVSTDVSQAEPVEIRYKKLLSLLTF ALQSIDNSHSMVGKLSRRIYLSSAR MVTTVPHVFSKLLEMLSVSSSTHFT RMRRRLMAIADEVEIAEAIQLGVED TLDGQQDSFLQASVPNNYLETTENS SPECTVHLEKTGKGLCATKLSASSE DISERLASISVGPSSSTTTTTTTTE QPKPMVQTKGRPHSQCLNSSPLSHH SQLMFPALSTPSSSTPSVPAGTATD VSKHRLQGFIPCRIPSASPQTQRKF SLQFHRNCPENKDSDKLSPVFTQSR PLPSSNIHRPKPSRPTPGNTSKQGD PSKNSMTLDLNSSSKCDDSFGCSSN SSNAVIPSDETVFTPVEEKCRLDVN TELNSSIEDLLEASMPSSDTTVTFK SEVAVLSPEKAENDDTYKDDVNHNQ KCKEKMEAEEEEALAIAMAMSASQD ALPIVPQLQVENGEDIIIIQQDTPE TLPGHTKAKQPYREDTEWLKGQQIG LGAFSSCYQAQDVGTGTLMAVKQVT YVRNTSSEQEEVVEALREEIRMMSH LNHPNIIRMLGATCEKSNYNLFIEW MAGGSVAHLLSKYGAFKESVVINYT EQLLRGLSYLHENQIIHRDVKGANL LIDSTGQRLRIADFGAAARLASKGT GAGEFQGQLLGTIAFMAPEVLRGQQ YGRSCDVWSVGCAIIEMACAKPPWN AEKHSNHLALIFKIASATTAPSIPS HLSPGLRDVALRCLELQPQDRPPSR ELLKHPVFRTTWTRTRPLEQKLISE EDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MAP3K1 mitogen-activated protein kinase kinase kinase 1 [ Homo sapiens (human) ]
• Official Symbol: MAP3K1
• Synonyms: MAP3K1; mitogen-activated protein kinase kinase kinase 1, E3 ubiquitin protein ligase; MEKK1, mitogen activated protein kinase kinase kinase 1; mitogen-activated protein kinase kinase kinase 1; MAPKKK1; MEKK; MEK kinase 1; MAP/ERK kinase kinase 1; MAPK/ERK kinase kinase 1; MEKK1; SRXY6; MEKK 1
• Gene ID: 4214
• mRNA Refseq: NM_005921
• Protein Refseq: NP_005912
• MIM: 600982
• UniProt ID: Q13233

Reviews

12/20/2020

The bands of proteins in Western Blotting experiments were very clear.

11/10/2020

The MAP3K1 protein is highly recommended for its exceptional performance in ELISA assays.

09/30/2017

Researchers can rely on the MAP3K1 protein to deliver precise results, enhancing the credibility and efficiency of their experiments.

Q&As

How is MAP3K1 protein expression measured in clinical settings?

MAP3K1 protein expression can be measured using immunohistochemistry (IHC) or Western blotting in clinical settings.

Are there any diseases associated with MAP3K1 protein mutations?

Yes, mutations in the MAP3K1 gene have been linked to several diseases, including breast cancer, lung cancer, and colon cancer.

What are the implications of MAP3K1 protein in drug resistance?

MAP3K1 protein has been implicated in the development of drug resistance in certain cancer types, highlighting its importance in personalized medicine and treatment response.

What are the potential diagnostic applications of MAP3K1 protein analysis?

MAP3K1 protein analysis can be used for diagnostic purposes to identify specific molecular subtypes of cancer and to predict disease prognosis.

How is MAP3K1 protein targeted in precision medicine approaches?

Precision medicine approaches may involve targeting MAP3K1 protein through specific inhibitors or monoclonal antibodies to inhibit its oncogenic activity.