Recombinant Human Mitogen-Activated Protein Kinase Kinase Kinase Kinase 1, GST-tagged

• Cat.No.: MAP4K1-1041H
• Product Overview: Recombinant human MAP4K1 (1-346) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.

Specification

• Cat. No.: MAP4K1-1041H
• Description: MAP4K1 or Hematopoietic progenitor kinase 1 is a hematopoietic cell-restricted member of the Ste20 serine/threonine kinase super family. MAP4K1 is also known as mitogen-activated protein kinase kinase kinase kinase 1(MAP4K1). MAP4K1 is a tissue-specific upstream activator of the MEKK/JNK/SAPK signaling pathway. MAP4K1 diminishes T cell receptor (TCR) signaling activity and T cell proliferation by phosphorylating the adaptor protein SLP-76.
• Applications: Kinase Assay; Western Blot
• Molecular Weight: 65 kDa
• Expression System: Sf9 insect cells using baculovirus.
• Species: Human
• Form: Recombinant protein stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25% glycerol.
• Specific Activity: 125 nmol/min/mg
• Purity: > 75 %
• Concentration: 0.1 ug/ul
• Sequences: 1-346
• Storage: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
• Pathways: Ceramide signaling pathway; Direct p53 effectors; EPHB forward signaling; Insulin Signaling; MAPK signaling pathway; TNF receptor signaling pathway

Gene Information

• Gene Name: MAP4K1 mitogen-activated protein kinase kinase kinase kinase 1 [ Homo sapiens ]
• Official Symbol: MAP4K1
• Synonyms: MAP4K1; HPK1; mitogen-activated protein kinase kinase kinase kinase 1; MAPK/ERK kinase kinase kinase 1; MEK kinase kinase 1; MEKKK 1; EC 2.7.11.1; hematopoietic progenitor kinase 1; Hematopoietic progenitor kinase; EC 2.7.11
• Gene ID: 11184
• mRNA Refseq: NM_007181
• Protein Refseq: NP_009112
• MIM: 601983
• UniProt ID: Q92918
• Chromosome Location: 19q13.1-q13.4
• Function: ATP binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; small GTPase regulator activity