Recombinant Human MAPT, His-tagged

• Cat.No.: MAPT-2874H
• Product Overview: Recombinant human Tau protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Specification

• Cat. No.: MAPT-2874H
• Description: Tau, also known as microtubule-associated protein tau (MAPT), is a protein that stabilizes microtubules. It is abundant in neurons in the central nervous system and is less common elsewhere. When this protein is defective, and no longer stabilizes microtubules properly, it can result in dementias, such as Alzheimer"s disease.
• Source: E.coli.
• Sequence: MGSSHHHHHH SSGLVPRGSH MAEPRQEFEV MEDHAGTYGL GDRKDQGGYT MHQDQEGDTD AGLKAEEAGI GDTPSLEDEA AGHVTQARMV SKSKDGTGSD DKKAKGADGK TKIATPRGAA PPGQKGQANA TRIPAKTPPA PKTPPSSGEP PKSGDRSGYS SPGSPGTPGS RSRTPSLPTP TREPKKVAV VRTPPKSPSS AKSRLQTAPV PMPDLKNVKS KIGSTENLKH QPGGGKVQII NKKLDLSNVQ SKCGSKDNIK HVPGGGSVQI VYKPVDLSKV TSKCGSLGNI HHKPGGGQVE VKSEKLDFKD RVQSKIGSLD NITHVPGGGN KKIETHKLTF RENAKAKTDH GAEIVYKSPV VSGDTSPRHL SNVSSTGSID MVDSPQLATL ADEVSASLAK QGL.
• Purity: > 90% by SDS – PAGE.
• MW: 42.1 kDa (403aa) confirmed by MALDI-TOF.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 10% glycerol, 2mM DTT, 50mM NaCl, 0.1mM PMSF.
• Concentration: 1mg/ml (determined by Bradford assay).
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: MAPT microtubule-associated protein tau [ Homo sapiens ]
• Synonyms: MAPT; microtubule-associated protein tau ; DDPAC, FLJ31424, FTDP-17, MAPTL, MGC138549, MSTD, MTBT1, MTBT2, PPND, TAU; G protein beta1/gamma2 subunit-interacting factor 1; PHF-tau; microtubule-associated protein tau, isoform 4; neurofibrillary tangle protein; paired helical filament-tau
• Gene ID: 4137
• mRNA Refseq: NM_001123066
• Protein Refseq: NP_001116538
• MIM: 157140
• UniProt ID: P10636
• Chromosome Location: 17q21
• Pathway: Alzheimer"s disease; MAPK signaling pathway
• Function: SH3 domain binding; apolipoprotein E binding; identical protein binding; lipoprotein particle binding; structural constituent of cytoskeleton

Reviews

07/22/2022

Western-blot experiments using MAPT as a target have shown highly reproducible results.

08/08/2020

This stability of MAPT is very good and can maintain its original properties even after multiple uses.

09/15/2019

MAPT's purity and consistency make it a top choice for Western-blot experiments.

Q&As

How to assess the degree of abnormal aggregation of MAPT protein?

The degree of abnormal aggregation of MAMAP proteins can be assessed by a variety of methods, such as immunohistochemistry, immunofluorescence staining, Western blot, etc. These methods can detect and quantify abnormally aggregated MAPT proteins.

What is the relationship between MAPT protein and nerve cytoskeleton?

The MAPT protein is closely related to the nerve cytoskeleton. It is involved in regulating axon formation and maintenance, cytosolic flow, and the normal function of neurons by binding and stabilizing microtubules.

What are the interactions of the MAPT protein with other proteins in the nervous system?

MAPT protein interacts with other proteins in the nervous system, such as α-synuclein, β-kinigin, etc. These interactions may be involved in regulating the structure and function of neurons.

Is there a mechanism that regulates the expression of MAPT protein?

Yes, the expression of MAPT protein is affected by a variety of regulatory mechanisms, including transcriptional regulation, splicing regulation, phosphorylation regulation, etc. These regulatory mechanisms are essential for maintaining normal MAPT protein levels and function.

What are the future directions for MAPT protein research?

Future research directions include further elucidating the mechanism of abnormal aggregation and phosphorylation of MAPT protein, exploring the pathogenesis of genetic diseases associated with MAPT gene, finding therapeutic strategies to intervene in abnormal aggregation of MATT, and developing biomarkers related to MAPT protein for early diagnosis and therapeutic monitoring.

How about the applications of MAPT protein in clinical diagnosis?

The MAPT protein plays an important role in clinical diagnosis. By measuring the level of the MAPT protein in the blood or cerebrospinal fluid, it can help identify different types of neurodegenerative diseases, such as Alzheimer's disease and prefrontal temporal dementia.