Recombinant Human MLF1 protein, His-tagged

• Cat.No.: MLF1-3438H
• Product Overview: Recombinant Human MLF1(1-268aa) fused with His tag at N-terminal was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-268 a.a.
• Description: MLF1 (Myeloid leukemia factor 1) belongs to MLF family, and is a widely expressed negative regulator of cell cycle progression functioning upstream of the tumor suppressor p53. This protein interferes with erythropoietin-induced erythroid terminal differentiation by preventing cells from exiting the cell cycle through suppression of CDKN1B/p27Kip1 levels. MLF1 normally functions in multi-potent progenitor cells, and its dysregulation may be somewhat responsible for leukemogenesis.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 5mM DTT, 200mM NaCl
• Molecular Mass: 32.8kDa (288aa), confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• AA Sequence: MGSSHHHHHH SSGLVPRGSHSFQTMDQMVS NMRNYMQKLE RNFGQLSVDP NGHSFCSSSV MTYSKIGDEP PKVFQASTQT RRAPGGIKET RKAMRDSDSG LEKMAIGHHI MFRMLNSSFE DDPFFSESIL AHRENMRQMI RSFSEPFGRD LLSISDGRGR AHNRRGHNDG EDSLTHTDVSHDRAHVIKKS KNKKTGDEEV NQEFINMNES DAHAFDEEWQ SEVLKYKPGR HNLGNTRMRS VGHENPGSRE LKRREKPQQS PAIEHGRRSNVLGDKLHIKG SSVKSNKK
• Purity: >85% by SDS - PAGE
• Storage: Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MLF1 myeloid leukemia factor 1 [ Homo sapiens ]
• Official Symbol: MLF1
• Synonyms: MLF1; myeloid leukemia factor 1; myeloid leukemia factor 1 variant 1; myeloid leukemia factor 1 variant 2; myeloid leukemia factor 1 variant 3; myelodysplasia-myeloid leukemia factor 1
• Gene ID: 4291
• mRNA Refseq: NM_022443
• Protein Refseq: NP_071888
• MIM: 601402
• UniProt ID: P58340
• Chromosome Location: 3q25
• Pathway: Transcriptional misregulation in cancer, organism-specific biosystem; Transcriptional misregulation in cancer, conserved biosystem
• Function: DNA binding; DNA binding; protein binding; protein domain specific binding