| Description : |
As the first member of membrane type (MT) MMPs, MMP-14, also known as MT1-MMP, plays an important role in extracellular matrix (ECM) remodeling by being able to degrade type I collagen, activate pro-MMP-2 and process cell adhesion molecules such as CD44 and integrin alpha V. MMP-14 is therefore a key enzyme in many physiological and pathological processes such as angiogenesis and tumor invasion. Structurally, MMP-14 consists of the following domains: a pro domain containing the furin cleavage site, a catalytic domain containing the zinc-binding site, a hinge region, a hemopexin-like domain, a transmembrane domain, and a cytoplamasic tail. Recombinant Human MMP-14 consists of the pro and catalytic domains, which can be activated by treatment with furin as described in Activity Assay Protocol. |
| Source : |
E.coli |
| Species : |
Human |
| Form : |
Supplied as a 0.2 μm filtered solution in 20 mM Tris-HCl, pH 7.4, 300 mM NaCl, 3 mM CaCl2,10 μM ZnCl2, with 30 % glycerol. |
| Molecular Mass : |
Approximately 29.6 kDa, a single non-glycosylated polypeptide chain containing 264 amino acids. |
| Protein length : |
264 |
| AA Sequence : |
ALASLGSAQSSSFSPEAWLQQYGYL PPGDLRTHTQRSPQSLSAAIAAMQK FYGLQVTGKADADTMKAMRRPRCGV PDKFGAEIKANVRRKRYAIQGLKWQ HNEITFCIQNYTPKVGEYATYEAIR KAFRVWESATPLRFREVPYAYIREG HEKQADIMIFFAEGFHGDSTPFDGE GGFLAHAYFPGPNIGGDTHFDSAEP WTVRNEDLNGNDIFLVAVHELGHAL GLEHSSDPSAIMAPFYQWMDTENFV LPDDDRRGIQQLYG |
| Endotoxin : |
Less than 1 EU/µg of rHuMMP-14 as determined by LAL method. |
| Purity : |
>95% by SDS-PAGE and HPLC analyses. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 centigrade as supplied. 3 months, -20 to -70 centigrade under sterile conditions after opening. |
