Recombinant Human MMP14 Protein, GST-tagged
Cat.No. : | MMP14-5423H |
Product Overview : | Human MMP14 full-length ORF ( AAH64803.1, 1 a.a. - 582 a.a.) recombinant protein with GST-tag at N-terminal. |
- Specification
- Gene Information
- Related Products
Description : | Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily; each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. This protein activates MMP2 protein, and this activity may be involved in tumor invasion. [provided by RefSeq |
Source : | Wheat Germ |
Species : | Human |
Tag : | GST |
Molecular Mass : | 92.3 kDa |
AA Sequence : | MSPAPRPSRCLLLPLLTLGTALASL GSAQSSSFSPEAWLQQYGYLPPGDL RTHTQRSPQSLSAAIAAMQKFYGLQ VTGKADADTMKAMRRPRCGVPDKFG AEIKANVRRKRYAIQGLKWQHNEIT FCIQNYTPKVGEYATYEAIRKAFRV WESATPLRFREVPYAYIREGHEKQA DIMIFFAEGFHGDSTPFDGEGGFLA HAYFPGPNIGGDTHFDSAEPWTVRN EDLNGNDIFLVAVHELGHALGLEHS SDPSAIMAPFYQWMDTENFVLPDDD RRGIQQLYGGESGFPTKMPPQPRTT SRPSVPDKPKNPTYGPNICDGNFDT VAMLRGEMFVFKERWFWRVRNNQVM DGYPMPIGQFWRGLPASINTAYERK DGKFVFFKGDKHWVFDEASLEPGYP KHIKELGRGLPTDKIDAALFWMPNG KTYFFRGNKYYRFNEELRAVDSEYP KNIKVWEGIPESPRGSFMGSDEVFT YFYKGNKYWKFNNQKLKVEPGYPKS ALRDWMGCPSGGRPDEGTEEETEVI IIEVDEEGGGAVSAAAVVLPVLLLL LVLAVGLAVFFFRRHGTPRRLLYCQ RSLLDKV |
Applications : | Enzyme-linked Immunoabsorbent Assay Western Blot (Recombinant protein) Antibody Production Protein Array |
Notes : | Best use within three months from the date of receipt of this protein. |
Storage : | Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
Storage Buffer : | 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
Gene Name : | MMP14 matrix metallopeptidase 14 (membrane-inserted) [ Homo sapiens ] |
Official Symbol : | MMP14 |
Synonyms : | MMP14; matrix metallopeptidase 14 (membrane-inserted); matrix metalloproteinase 14 (membrane inserted); matrix metalloproteinase-14; membrane type 1 metalloprotease; MT1 MMP; membrane-type matrix metalloproteinase 1; membrane-type-1 matrix metalloproteinase; matrix metalloproteinase 14 (membrane-inserted); 1; MMP-14; MMP-X1; MT-MMP; MT1MMP; MTMMP1; MT1-MMP; MT-MMP 1; |
Gene ID : | 4323 |
mRNA Refseq : | NM_004995 |
Protein Refseq : | NP_004986 |
MIM : | 600754 |
UniProt ID : | P50281 |
Products Types
◆ Recombinant Protein | ||
MMP14-181H | Recombinant Human MMP14 Protein, His-tagged | +Inquiry |
MMP14-5599M | Recombinant Mouse MMP14 Protein, His (Fc)-Avi-tagged | +Inquiry |
MMP14-3369R | Recombinant Rat MMP14 Protein, His (Fc)-Avi-tagged | +Inquiry |
Mmp14-183M | Recombinant Mouse Mmp14 Protein, His-tagged | +Inquiry |
MMP14-571H | Recombinant Human MMP14 Protein, MYC/DDK-tagged | +Inquiry |
◆ Lysates | ||
MMP14-4279HCL | Recombinant Human MMP14 293 Cell Lysate | +Inquiry |
◆ Assay kits | ||
Kit-2123 | MMP-14 Inhibitor Screening Kit | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (7)
Ask a questionMMP14, also known as matrix metalloproteinase 14, is primarily expressed by cells of mesenchymal origin, such as fibroblasts and endothelial cells. Its expression is tightly regulated at multiple levels, including transcriptional, post-transcriptional, and post-translational mechanisms. Transcriptional regulation involves the binding of various transcription factors, such as AP-1 and SP-1, to the MMP14 promoter region. Additionally, microRNAs and RNA-binding proteins can modulate its mRNA stability and translation.
MMP14 plays a crucial role in cancer progression and metastasis. It facilitates tumor invasion by degrading components of the extracellular matrix, promoting cancer cell migration, and aiding in angiogenesis. MMP14 also influences tumor cell behavior by interacting with other proteins involved in cell signaling pathways and epithelial-mesenchymal transition. Its overexpression is associated with poor prognosis in various types of cancer.
Overexpression of MMP14 has been strongly associated with tumor progression and poor prognosis in various types of cancer. Increased levels of MMP14 lead to enhanced degradation of the ECM, promoting tumor cell invasion and metastasis. Additionally, MMP14 can activate other matrix metalloproteinases (MMPs), further amplifying the proteolytic activity and facilitating tumor growth and angiogenesis. Moreover, MMP14 has been implicated in tumor-associated angiogenesis and immunosuppression, contributing to tumor progression and metastasis.
Targeting MMP14 holds potential for therapeutic interventions in cancer and other diseases. Strategies such as selective inhibition of MMP14 activity, antibody-based therapies, and gene silencing techniques are being explored. However, the development of MMP14-targeted therapies faces challenges, including the need for specificity, avoiding off-target effects, and optimizing delivery methods to reach the tumor microenvironment.
Yes, there are endogenous inhibitors of MMP14, known as tissue inhibitors of metalloproteinases (TIMPs). TIMPs bind to the active site of MMP14 and prevent its activity. TIMP-2 is a specific inhibitor of MMP14 and forms a complex with MMP14, regulating its enzymatic activity. The balance between MMP14 and TIMPs is crucial for maintaining tissue homeostasis and preventing excessive extracellular matrix degradation. Dysregulation of this balance can contribute to pathological conditions, including cancer progression.
MMP14 plays a crucial role in tissue remodeling processes, such as embryogenesis, wound healing, and tissue repair. It is essential for the degradation and turnover of extracellular matrix components during tissue restructuring. MMP14 also influences cell behavior and migration during tissue development and regeneration. Additionally, it participates in the modulation of immune responses and angiogenesis, which are crucial for proper tissue remodeling.
MMP14 possesses a catalytic domain responsible for substrate cleavage. It predominantly cleaves components of the extracellular matrix, such as collagen type I and type II. The interaction between MMP14 and its substrates occurs through specific binding sites within the substrate proteins. This interaction is facilitated by the hemopexin-like domain of MMP14, which acts as a docking site for substrate recognition and binding.
Customer Reviews (3)
Write a reviewThe versatility of this reagent is evident in its capacity to cater to a plethora of sample types, offering a broad array of potential applications.
Backed by its user-friendly nature, this protein reagent saves me considerable time and effort, making it highly suitable for fast-paced laboratory work.
Brimming with enchantment, this experimental reagent illuminates your scientific odyssey.
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