Recombinant Human MPI Protein, MYC/DDK-tagged, C13 and N15-labeled

• Cat.No.: MPI-162H
• Product Overview: MPI MS Standard C13 and N15-labeled recombinant protein (NP_002426) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: Phosphomannose isomerase catalyzes the interconversion of fructose-6-phosphate and mannose-6-phosphate and plays a critical role in maintaining the supply of D-mannose derivatives, which are required for most glycosylation reactions. Mutations in the MPI gene were found in patients with carbohydrate-deficient glycoprotein syndrome, type Ib. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
• Source: HEK293
• Species: Human
• Tag: Myc/DDK
• Molecular Mass: 46.7 kDa
• AA Sequence: MAAPRVFPLSCAVQQYAWGKMGSNS EVARLLASSDPLAQIAEDKPYAELW MGTHPRGDAKILDNRISQKTLSQWI AENQDSLGSKVKDTFNGNLPFLFKV LSVETPLSIQAHPNKELAEKLHLQA PQHYPDANHKPEMAIALTPFQGLCG FRPVEEIVTFLKKVPEFQFLIGDEA ATHLKQTMSHDSQAVASSLQSCFSH LMKSEKKVVVEQLNLLVKRISQQAA AGNNMEDIFGELLLQLHQQYPGDIG CFAIYFLNLLTLKPGEAMFLEANVP HAYLKGDCVECMACSDNTVRAGLTP KFIDVPTLCEMLSYTPSSSKDRLFL PTRSQEDPYLSIYDPPVPDFTIMKT EVPGSVTEYKVLALDSASILLMVQG TVIASTPTTQTPIPLQRGGVLFIGA NESVSLKLTEPKDLLIFRACCLLTR TRPLEQKLISEEDLAANDILDYKDD DDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: MPI mannose phosphate isomerase [ Homo sapiens (human) ]
• Official Symbol: MPI
• Synonyms: MPI; mannose phosphate isomerase; CDG1B; PMI; PMI1; mannose-6-phosphate isomerase; phosphohexomutase; phosphomannose isomerase 1; EC 5.3.1.8
• Gene ID: 4351
• mRNA Refseq: NM_002435
• Protein Refseq: NP_002426
• MIM: 154550
• UniProt ID: P34949