Recombinant Human MRE11 protein, His & GST-tagged

• Cat.No.: MRE11-1747H
• Product Overview: Recombinant Human MRE11 aa. (Pro375~Lys609 (Accession # P49959)) fused with N-terminal His & GST tag was produced in E. coli cells.

Specification

• Description: This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
• Source: E. coli
• Species: Human
• Tag: His & GST
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 56.8kDa
• Protein length: Pro375~Lys609 (Accession # P49959)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 6.5

Gene Information

• Gene Name: MRE11 MRE11 homolog, double strand break repair nuclease [ Homo sapiens (human) ]
• Official Symbol: MRE11
• Synonyms: MRE11; MRE11 homolog, double strand break repair nuclease; ATLD; HNGS1; MRE11A; MRE11B; double-strand break repair protein MRE11; double-strand break repair protein MRE11A; AT-like disease; DNA recombination and repair protein; MRE11 double strand break repair nuclease A; MRE11 homolog 1; MRE11 homolog A, double strand break repair nuclease; MRE11 homolog, double strand break repair nuclease A; MRE11 meiotic recombination 11 homolog A; MRE11 meiotic recombination 11-like protein A; endo/exonuclease Mre11; meiotic recombination 11 homolog 1; meiotic recombination 11 homolog A
• Gene ID: 4361
• mRNA Refseq: NM_005590.3
• Protein Refseq: NP_005581.2
• UniProt ID: P49959