Recombinant Full Length Human MRE11 Protein, C-Flag-tagged

• Cat.No.: MRE11-651HFL
• Product Overview: Recombinant Full Length Human MRE11 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: This gene encodes a nuclear protein involved in homologous recombination, telomere length maintenance, and DNA double-strand break repair. By itself, the protein has 3' to 5' exonuclease activity and endonuclease activity. The protein forms a complex with the RAD50 homolog; this complex is required for nonhomologous joining of DNA ends and possesses increased single-stranded DNA endonuclease and 3' to 5' exonuclease activities. In conjunction with a DNA ligase, this protein promotes the joining of noncomplementary ends in vitro using short homologies near the ends of the DNA fragments. This gene has a pseudogene on chromosome 3. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 80.4 kDa
• AA Sequence: MSTADALDDENTFKILVATDIHLGF MEKDAVRGNDTFVTLDEILRLAQEN EVDFILLGGDLFHENKPSRK TLHTCLELLRKYCMGDRPVQFEILS DQSVNFGFSKFPWVNYQDGNLNISI PVFSIHGNHDDPTGADALCA LDILSCAGFVNHFGRSMSVEKIDIS PVLLQKGSTKIALYGLGSIPDERLY RMFVNKKVTMLRPKEDENSW FNLFVIHQNRSKHGSTNFIPEQFLD DFIDLVIWGHEHECKIAPTKNEQQL FYISQPGSSVVTSLSPGEAV KKHVGLLRIKGRKMNMHKIPLHTVR QFFMEDIVLANHPDIFNPDNPKVTQ AIQSFCLEKIEEMLENAERE RLGNSHQPEKPLVRLRVDYSGGFEP FSVLRFSQKFVDRVANPKDIIHFFR HREQKEKTGEEINFGKLITK PSEGTTLRVEDLVKQYFQTAEKNVQ LSLLTERGMGEAVQEFVDKEEKDAI EELVKYQLEKTQRFLKERHI DALEDKIDEEVRRFRETRQKNTNEE DDEVREAMTRARALRSQSEESASAF SADDLMSIDLAEQMANDSDD SISAATNKGRGRGRGRRGGRGQNSA SRGGSQRGRADTGLETSTRSRNSKT AVSASRNMSIIDAFKSTRQQ PSRNVTTKNYSEVIEVDESDVEEDI FPTTSKTDQRWSSTSSSKIMSQSQV SKGVDFESSEDDDDDPFMNT
  SSLRRNRRTRTRPLEQKLISEEDLA ANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Homologous recombination, Non-homologous end-joining

Gene Information

• Gene Name: MRE11 MRE11 homolog, double strand break repair nuclease [ Homo sapiens (human) ]
• Official Symbol: MRE11
• Synonyms: ATLD; HNGS1; MRE11A; MRE11B
• Gene ID: 4361
• mRNA Refseq: NM_005591.4
• Protein Refseq: NP_005582.1
• MIM: 600814
• UniProt ID: P49959