| Description : |
Mesothelin (MSLN) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum. It is overexpressed in mesotheliomas, ovarian and pancreatic cancers as well as in specific squamous cell carcinomas. The MSLN gene encodes a precursor protein that is cleaved into two products, megakaryocyte-potentiating factor (MPF) and MSLN. MPF is secreted and functions as a cytokine that can stimulate colony formation in bone marrow megakaryocytes. The cleaved form of MSLN remains GPI-anchored on cell-surface and may function as a cell adhesion molecule. Alternative splicing results in multiple transcript variants. Two variant forms exist; variant 1 has an eight amino acid insertion and is rarely expressed, while variant 2 is a truncated form found in cancers but rarely detected in normal individuals. MSLN interacts with CA125/MUC16 and potentiates megakaryocyte colony formation in vitro. MSLN is secreted by several mesothelioma cell lines and is frequently elevated in the blood of patients with mesothelioma. Measurement of this protein may be useful in following the response of mesothelioma to treatment. |
| Source : |
HEK293 |
| Species : |
Human |
| Tag : |
Fc |
| Form : |
Supplied at 0.5 mg/ml in sterile PBS pH7.4 (carrier & preservative free). |
| Bio-activity : |
Binds to CA125/MUC16 and anti-MSLN monoclonal antibody, human IgG1 with high affinity. Blocks MSLN's binding to CA125/MUC16. |
| Molecular Mass : |
Calculated molecular mass (kDa): 60.1; Estimated by SDS-PAGE under reducing condition (kDa): 65-70 |
| Protein length : |
Glu296 - Ser592 |
| AA Sequence : |
EVEKTACPSGKKAREIDESLIFYKK WELEACVDAALLATQMDRVNAIPFT YEQLDVLKHKLDELYPQGYPESVIQ HLGYLFLKMSPEDIRKWNVTSLETL KALLEVNKGHEMSPQVATLIDRFVK GRGQLDKDTLDTLTAFYPGYLCSLS PEELSSVPPSSIWAVRPQDLDTCDP RQLDVLYPKARLAFQNMNGSEYFVK IQSFLGGAPTEDLKALSQQNVSMDL ATFMKLRTDAVLPLTVAEVQKLLGP HVEGLKAEERHRPVRDWILRQRQDD LDTLGLGLQGGIPNGYLVLDLSTTE NLYFQGSTGTHTCPPCPAPELLGGP SVFLFPPKPKDTLMISRTPEVTCVV VDVSHEDPEVKFNWYVDGVEVHNAK TKPREEQYNSTYRVVSVLTVLHQDW LNGKEYKCKVSNKALPAPIEKTISK AKGQPREPQVYTLPPSREEMTKNQV SLTCLVKGFYPSDIAVEWESNGQPE NNYKTTPPVLDSDGSFFLYSKLTVD KSRWQQGNVFSCSVMHEALHNHYTQ KSLSLSPGK |
| Endotoxin : |
<0.1 EU per 1 μg of purified recombinant protein determined by the LAL method |
| Purity : |
>95% judged by SDS-PAGE under reducing condition |
| Storage : |
Upon receipt, centrifuge the product briefly before opening the vial. Store small aliquots at the temperature below -20 centigrade for long-term storage and the product is stable for at least 3 months. Avoid repeapted free-thaw cycles. |
| Shipping : |
Ship at 4 centigrade |
