Recombinant Human MYL5, His-tagged

• Cat.No.: MYL5-3622H
• Product Overview: Recombinant human MYL5 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques (1-132 aa).

Specification

• Species: Human
• Source: Human
• Tag: His
• Description: Myosin regulatory light chain 5, also known as MYL5, is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia. Reconstitution of myosin with regulatory light chain 5 or alkali light chain increases filament velocity to intermediate rates, and readdition of both classes of light chains fully restores the original sliding velocity.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl
• Molecular Weight: 17.4 kDa (156aa), confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 0.5 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MGSHMDQNRD GFIDKEDLKD TYASLGKTNV KDDELDAMLK EASGPINFTM FLNLFGEKLS GTDAEETILN AFKMLDPDGK GKINKEYIKR LLMSQADKMT AEEVDQMFQF ASIDVAGNLD YKALSYVITH GEEKEE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Pathways: Focal adhesion; Leukocyte transendothelial migration; Regulation of actin cytoskeleton; Tight junction

Gene Information

• Gene Name: MYL5 myosin, light chain 5, regulatory [ Homo sapiens ]
• Official Symbol: MYL5
• Synonyms: MYL5; myosin, light chain 5, regulatory; myosin light chain 5; MYLC2; myLC-2; MyLC-2; OTTHUMP00000217187; OTTHUMP00000217188; OTTHUMP00000217190; OTTHUMP00000217193; myosin regulatory light chain 5; myosin, light polypeptide 5, regulatory; superfast myosin regulatory light chain 2
• Gene ID: 4636
• mRNA Refseq: NM_002477
• Protein Refseq: NP_002468
• MIM: 160782
• UniProt ID: Q02045
• Chromosome Location: 4p16.3
• Function: calcium ion binding; structural constituent of muscle