Recombinant Human Myosin, Light Chain 5, Regulatory, His-tagged

• Cat.No.: MYL5-6875H
• Product Overview: Recombinant human MYL5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography. MW: 22.1 kDa.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 1-173aa
• Description: MYL5 is a hexameric ATPase cellular motor protein. Myosin is composed of two heavy chains, two nonphosphorylatable alkali light chains, and two phosphorylatable regulatory light chains. MYL5 is a regulatory light chain and is expressed in fetal muscle and in adult retina, cerebellum, and basal ganglia.
• Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol 0.1M NaCl,1mM DTT, 0.1mM PMSF.
• Molecular Mass: 22.1 kDa (197aa), confirmed by MALDI-TOF.
• AA Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMASRKT KKKEGGALRA QRASSNVFSN FEQTQIQEFK EAFTLMDQNR DGFIDKEDLK DTYASLGKTN VKDDELDAML KEASGPINFT MFLNLFGEKL SGTDAEETIL NAFKMLDPDG KGKINKEYIK RLLMSQADKM TAEEVDQMFQ FASIDVAGNL DYKALSYVIT HGEEKEE
• Purity: >90% by SDS - PAGE
• Applications: SDS-PAGE
• Storage: Can be stored at 4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/ml (determined by Bradford assay)

Gene Information

• Gene Name: MYL5 myosin, light chain 5, regulatory [ Homo sapiens ]
• Official Symbol: MYL5
• Synonyms: MYL5; myosin, light chain 5, regulatory; myosin, light polypeptide 5, regulatory; myosin light chain 5; MYLC2; myLC-2; myosin regulatory light chain 5; superfast myosin regulatory light chain 2
• Gene ID: 4636
• mRNA Refseq: NM_002477
• Protein Refseq: NP_002468
• MIM: 160782
• UniProt ID: Q02045
• Chromosome Location: 4p16
• Pathway: Focal adhesion, organism-specific biosystem; Focal adhesion, conserved biosystem; Leukocyte transendothelial migration, organism-specific biosystem; Leukocyte transendothelial migration, conserved biosystem; Regulation of actin cytoskeleton, organism-specific biosystem; Regulation of actin cytoskeleton, conserved biosystem; Tight junction, organism-specific biosystem
• Function: calcium ion binding; structural constituent of muscle