Recombinant Human MYL6 293 Cell Lysate

• Cat.No.: MYL6-4024HCL

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Antigen standard for myosin, light chain 6, alkali, smooth muscle and non-muscle (MYL6), transcript variant 2 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: MYL6 myosin, light chain 6, alkali, smooth muscle and non-muscle [ Homo sapiens ]
• Official Symbol: MYL6
• Synonyms: MYL6; myosin, light chain 6, alkali, smooth muscle and non-muscle; myosin, light polypeptide 6, alkali, smooth muscle and non muscle; myosin light polypeptide 6; ESMLC; MLC1SM; MLC3NM; myosin light chain A3; 17 kDa myosin light chain; myosin light chain alkali 3; myosin, light polypeptide 6, alkali, smooth muscle and non-muscle; LC17; LC17A; LC17B; MLC-3; MLC3SM; LC17-GI; LC17-NM
• Gene ID: 4637
• mRNA Refseq: NM_079423
• Protein Refseq: NP_524147
• MIM: 609931
• UniProt ID: P60660
• Chromosome Location: 12q13.13
• Pathway: Axon guidance, organism-specific biosystem; Developmental Biology, organism-specific biosystem; Focal Adhesion, organism-specific biosystem; Muscle contraction, organism-specific biosystem; Sema4D in semaphorin signaling, organism-specific biosystem; Sema4D induced cell migration and growth-cone collapse, organism-specific biosystem; Semaphorin interactions, organism-specific biosystem
• Function: actin-dependent ATPase activity; calcium ion binding; motor activity; protein binding; structural constituent of muscle