Recombinant Human NGF

• Cat.No.: NGF-495H
• Product Overview: Recombinant Human Beta-NGF produced in CHO cells is a noncovalently disulfide-linked homodimer, of two 118 amino acids polypeptide monomers and having a molecular mass of 26.5 kDa.

Specification

• Species: Human
• Source: CHO
• Tag: Non
• Description: Beta-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Beta-NGF is a potent neurotrophic factor that signals through its receptor beta-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Beta-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. The functional form of human beta-NGF is a homodimer and the three disulfide bonds are required for biological activity.
• Biological Activity: The ED50 as determined by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1 ng/ml, corresponding to a Specific Activity of 1.0 x 10⁶ IU/mg.
• Purity: Greater than 95% as determined by (a) Analysis by RP-HPLC; (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.
• Molecular Weight: The molecular mass was determined and found to be 13203 Da by Mass Spectrum.
• Amino-Acid Sequence: The full sequence was verified by MS/MS.
• Endotoxin: Less than 0.08 ng/µg (0.8 IEU/µg) of rHuβ-NGF.
• Form: The protein was lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.2, 200mM NaCl buffer.
• Reconstitution: It is recommended to reconstitute the lyophilized rHuβ-NGF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Storage: Lyophilized rHuβ-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuβ-NGF should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Gene Information

• Gene Name: NGF nerve growth factor (beta polypeptide) [ Homo sapiens ]
• Official Symbol: NGF
• Synonyms: NGF; nerve growth factor (beta polypeptide); NGFB; HSAN5; Beta-NGF; MGC161428; Beta-nerve growth factor; nerve growth factor, beta subunit; nerve growth factor, beta polypeptide; beta-nerve growth factor; OTTHUMP00000013653; MGC161426
• Gene ID: 4803
• mRNA Refseq: NM_002506
• Protein Refseq: NP_002497
• MIM: 162030
• UniProt ID: P01138
• Chromosome Location: 1p13.1
• Function: growth factor activity; rve growth factor receptor binding; receptor signaling protein activity