Active Recombinant Human PDGFD protein, His-tagged

• Cat.No.: PDGFD-2572H
• Product Overview: Active Recombinant Human PDGFD protein(Q9GZP0)(19-370 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 19-370 aa
• Form: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Platelet-derived growth factor D (PDGFD) is a protein that in humans is encoded by the PDGFD gene. The protein encoded by this gene is a member of the platelet-derived growth factor family. PDGF plays a significant role in blood vessel formation, the growth of blood vessels from already-existing blood vessel tissue, mitogenesis. PDGF also plays a role in embryonic development, cell proliferation, cell migration, and angiogenesis. Besides, Macrophage Erythroblast Attacher (MAEA) has been identified as an interactor of PDGFD, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PDGFD and recombinant human MAEA. Briefly, PDGFD were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to MAEA-coated microtiter wells and incubated for 2h at 37°C. Wells were washed with PBST and incubated for 1h with anti-PDGFD pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50µL stop solution to the wells and read at 450nm immediately. The binding activity of PDGFD and MAEA was shown, and this effect was in a dose dependent manner.
• Molecular Mass: 44.4kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90%
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (PH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: PDGFD platelet derived growth factor D [ Homo sapiens ]
• Official Symbol: PDGFD
• Synonyms: PDGFD; platelet derived growth factor D; platelet-derived growth factor D; IEGF; MSTP036; SCDGF B; spinal cord derived growth factor B; PDGF-D; iris-expressed growth factor; spinal cord-derived growth factor B; spinal cord-derived growth factor-B; SCDGFB; SCDGF-B; MGC26867
• Gene ID: 80310
• mRNA Refseq: NM_025208
• Protein Refseq: NP_079484
• MIM: 609673
• UniProt ID: Q9GZP0