Active Recombinant Human PDGFC protein, His-tagged

• Cat.No.: PDGFC-441H
• Product Overview: Recombinant Human PDGFC protein, His-tagged(NP_057289)(235-345 aa), fused with His tag, was expressed in E. coli.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Protein Length: 235-345 aa
• Form: Lyophilized from 0.2 µm filtered concentrated solution in 30 % Acetonitrile and 0.1 % TFA.
• Bio-activity: Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. The ED50 for this effect is 70-350 ng/mL.
• Molecular Mass: Approximately 27 kDa, a disulfide-linked homodimer of two 117 amino acid, C-terminal polyhistidine tagged proteins.
• Endotoxin: Less than 0.1 EU/µg of rHuPDGF-CC as determined by LAL method.
• Purity: > 97 % by SDS-PAGE analyses.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. - 12 months from date of receipt, -20 to -70 °C as supplied. - 1 month, 2 to 8 °C under sterile conditions after reconstitution. - 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Gene Information

• Gene Name: PDGFC platelet derived growth factor C [ Homo sapiens ]
• Official Symbol: PDGFC
• Synonyms: PDGFC; platelet derived growth factor C; platelet-derived growth factor C; fallotein; SCDGF; PDGF-C; VEGF-E; spinal cord-derived growth factor; secretory growth factor-like protein; FALLOTEIN
• Gene ID: 56034
• mRNA Refseq: NM_016205
• Protein Refseq: NP_057289
• MIM: 608452
• UniProt ID: Q9NRA1