Recombinant Human POLA1 protein

• Cat.No.: POLA-938H
• Product Overview: Recombinant Human POLA1 protein was isolated from HeLa cells.

Specification

• Description: Human DNA Polymerase Alpha is a replication enzyme used to test the toxicity of drugs on key human replication systems. The simplest DNA Polymerase known in both size and catalysis. Replication enzyme involved in initiation of DNA replication in eukaryotes. A multi-subunit complex, which inhibits DNA polymerase and primase activities.
• Source: HeLa
• Species: Human
• Form: 20 mM Tris-HCl (pH 8.0), 0.25 mM EDTA, 50 mM NaCl, 1 mM β-mercaptoethanol, 0.1% Triton X-100™, 50% (v/v) glycerol
• Unit Definition: One unit is the amount of enzyme required to incorporate 1 nmole of total nucleotide into acid-insoluble form in 60 minutes at 37 centigrade.
• Applications: 1. Used to test in vitro replication capabilities along with other enzymes.2. Used to test the toxicity of drugs on key human replication systems.3. Used in DNA replication experiments.
• Storage: Store at -80 centigrade long term;Store at -20 centigrade short term.
• Publication:
  Mechanism of action of the atypical retinoid ST1926 in colorectal cancer: DNA damage and DNA polymerase α (2018)
  Antitumor activity of novel POLA1-HDAC11 dual inhibitors (2022)

Gene Information

• Gene Name: POLA1 polymerase (DNA directed), alpha 1, catalytic subunit [ Homo sapiens ]
• Official Symbol: POLA1
• Synonyms: POLA1; polymerase (DNA directed), alpha 1, catalytic subunit; POLA, polymerase (DNA directed), alpha , polymerase (DNA directed), alpha 1; DNA polymerase alpha catalytic subunit; p180; DNA polymerase alpha p180 subunit; polymerase (DNA-directed), alpha (70kD); DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha catalytic subunit p180; POLA; DKFZp686K1672
• Gene ID: 5422
• mRNA Refseq: NM_016937
• Protein Refseq: NP_058633
• MIM: 312040
• UniProt ID: P09884
• Chromosome Location: Xp22.1-p21.3
• Pathway: Activation of the pre-replicative complex, organism-specific biosystem; Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; DNA Replication, organism-specific biosystem; DNA Replication, organism-specific biosystem; DNA Replication Pre-Initiation, organism-specific biosystem
• Function: DNA binding; DNA binding; contributes_to DNA primase activity; DNA-directed DNA polymerase activity; DNA-directed DNA polymerase activity; chromatin binding; double-stranded DNA binding; metal ion binding; nucleoside binding; nucleotide binding; nucleotidyltransferase activity; protein binding; protein heterodimerization activity; purine nucleotide binding; pyrimidine nucleotide binding; transferase activity