"PRAME" Related Products

Recombinant Human PRAME Protein, His/GST-tagged

Cat.No.: PRAME-724H
Product Overview: Recombinant Human PRAME(Asn416~Leu502) fused with His/GST tag at N-terminal was expressed in E. coli.
Description: This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants.
Source: E. coli
Species: Human
Tag : His/GST
Form: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass: 36.9kDa
Protein length: Asn416~Leu502
Endotoxin: <1.0EU per 1µg (determined by the LAL method)
Purity: > 92%
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
Reconstitution: Reconstitute in PBS or others.
Gene Name: PRAME preferentially expressed antigen in melanoma [ Homo sapiens ]
Official Symbol: PRAME
Synonyms: PRAME; preferentially expressed antigen in melanoma; MAPE; melanoma antigen preferentially expressed in tumors; cancer/testis antigen 130; CT130; OIP-4; opa-interacting protein 4; Opa-interacting protein OIP4; preferentially expressed antigen of melanoma; OIP4;
Gene ID: 23532
mRNA Refseq: NM_006115
Protein Refseq: NP_006106
MIM: 606021
UniProt ID: P78395

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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