Recombinant Human PRDX1 Protein (Met1-Lys199), His-tagged

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Recombinant Human PRDX1 Protein (Met1-Lys199), His-tagged

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Cat.No. : PRDX1-504H
Product Overview : Recombinant Human PRDX1 Protein (NP_002565.1) (Met1-Lys199) was produced by E. coli expression system. This protein was expressed with a polyhistidine tag at the C-terminus.
Description : Peroxiredoxin-1, also known as Thioredoxin peroxidase 2, Natural killer cell-enhancing factor A, PRDX1, and PAGA, is a member of the ahpC/TSA family. Peroxiredoxin-1 is constitutively expressed in most human cells. It is induced to higher levels upon serum stimulation in untransformed and transformed cells. Peroxiredoxins (PRDXs) are a family of antioxidant enzymes that are also known as scavengers of peroxide in mammalian cells. The overexpression of Peroxiredoxin-1, which is one of the peroxiredoxins that is a ubiquitously expressed protein, was related to a poor prognosis in several types of human cancers. Peroxiredoxin-1 is involved in redox regulation of the cell. It reduces peroxides with reducing equivalents provided through the thioredoxin system but not from glutaredoxin and may play an important role in eliminating peroxides generated during metabolism. Peroxiredoxin-1 Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2. The reduced Peroxiredoxin-1 expression is an important factor in esophageal squamous cancer progression and could serve as a useful prognostic marker.
Source : E. coli
Species : Human
Tag : His
Predicted N Terminal : Met 1
Form : Lyophilized from sterile PBS, 10% glycerol, 0.1mM DTT, pH 7.5. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Molecular Mass : The recombinant human PRDX1 comprises 205 amino acids and has a predicted molecular mass of 22.9 kDa as estimated in SDS-PAGE under reducing conditions.
Protein length : Met1-Lys199
Purity : > 97 % as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : PRDX1 peroxiredoxin 1 [ Homo sapiens ]
Official Symbol : PRDX1
Synonyms : PRDX1; peroxiredoxin 1; PAGA; peroxiredoxin-1; NKEFA; NKEF-A; PAG; PAGB; PRX1; PRXI; MSP23; TDPX2;
Gene ID : 5052
mRNA Refseq : NM_001202431
Protein Refseq : NP_001189360
MIM : 176763
UniProt ID : Q06830

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