Recombinant Human PRDX2 cell lysate

• Cat.No.: PRDX2-564HCL
• Product Overview: Human PRDX2 / Peroxiredoxin-2 / NKEFB derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: PRDX2 peroxiredoxin 2 [ Homo sapiens ]
• Official Symbol: PRDX2
• Synonyms: PRDX2; peroxiredoxin 2; TDPX1; peroxiredoxin-2; MGC4104; natural killer enhancing factor B; NKEFB; PRP; PRX2; PRXII; thiol specific antioxidant 1; thioredoxin peroxidase 1; thioredoxin dependent peroxide reductase 1; torin; TSA; NKEF-B; thiol-specific antioxidant 1; thiol-specific antioxidant protein; natural killer cell-enhancing factor B; thioredoxin-dependent peroxide reductase 1; TPX1
• Gene ID: 7001
• mRNA Refseq: NM_005809
• Protein Refseq: NP_005800
• MIM: 600538
• UniProt ID: P32119
• Chromosome Location: 19p13.2
• Pathway: Selenium Pathway, organism-specific biosystem
• Function: antioxidant activity; antioxidant activity; oxidoreductase activity; thioredoxin peroxidase activity; thioredoxin peroxidase activity

Reviews

02/01/2022

I am eager to embark on this scientific journey, empowered by the high-quality PRDX2 protein and the manufacturer's unparalleled assistance.

12/16/2020

The manufacturer's prompt and reliable technical support serves as an invaluable resource, reinforcing my confidence in the PRDX2 protein's effectiveness and enabling smooth progress in my experiments.

11/01/2017

Its remarkable purity and functional attributes make it an optimal choice for my research, ensuring accurate and reliable outcomes.

Q&As

How does PRDX2 contribute to the field of cancer research and treatment?

PRDX2's role in maintaining cellular redox balance implicates it in cancer progression. Modulating PRDX2 levels may offer avenues for developing novel cancer therapies.

Are there any side effects associated with targeting PRDX2 for therapeutic purposes?

As with any targeted therapy, potential side effects need thorough investigation. Modulating PRDX2 levels could impact normal cellular functions, and careful assessment is required.

Is there evidence supporting the use of PRDX2 as a prognostic marker in certain diseases?

Some studies suggest that PRDX2 levels may have prognostic value in diseases associated with oxidative stress, providing insights into disease progression.

Can PRDX2 be a target for drug development, and what challenges might be associated with this?

Yes, PRDX2 is a potential drug target, but challenges include developing specific modulators and understanding the protein's complex regulatory mechanisms.

Are there any specific diagnostic tests or biomarkers related to PRDX2 protein levels?

Research suggests that altered levels of PRDX2 could serve as a biomarker in diseases associated with oxidative stress, offering potential diagnostic applications.