Recombinant Human PREX2 Protein, Myc/DDK-tagged, C13 and N15-labeled PREX2-6179H

Specification Gene Information Related Products

Cat.No. :	PREX2-6179H
Product Overview :	PREX2 MS Standard C13 and N15-labeled recombinant protein (NP_079146) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.
Description :	The protein encoded by this gene belongs to the phosphatidylinositol 3,4,5-trisphosphate (PIP3)-dependent Rac exchanger (PREX) family, which are Dbl-type guanine-nucleotide exchange factors for Rac family small G proteins. Structural domains of this protein include the catalytic diffuse B-cell lymphoma homology and pleckstrin homology (DHPH) domain, two disheveled, EGL-10, and pleckstrin homology (DEP) domains, two PDZ domains, and a C-terminal inositol polyphosphate-4 phosphatase (IP4P) domain that is found in one of the isoforms. This protein facilitates the exchange of GDP for GTP on Rac1, allowing the GTP-bound Rac1 to activate downstream effectors. Studies also show that the pleckstrin homology domain of this protein interacts with the phosphatase and tensin homolog (PTEN) gene product to inhibit PTEN phosphatase activity, thus activating the phosphoinositide-3 kinase (PI3K) signaling pathway. Conversely, the PTEN gene product has also been shown to inhibit the GEF activity of this protein. This gene plays a role in insulin-signaling pathways, and either mutations or overexpression of this gene have been observed in some cancers.
Source :	HEK293
Species :	Human
Tag :	Myc/DDK
Molecular Mass :	182.4 kDa
AA Sequence :	MSEDSRGDSR AESAKDLEKQ LRLRVCVLSE LQKTERDYVG TLEFLVSAFL HRMNQCAASK VDKNVTEETV KMLFSNIEDI LAVHKEFLKV VEECLHPEPN AQQEVGTCFL HFKDKFRIYD EYCSNHEKAQ KLLLELNKIR TIRTFLLNCM LLGGRKNTDV PLEGYLVTPI QRICKYPLIL KELLKRTPRK HSDYAAVMEA LQAMKAVCSN INEAKRQMEK LEVLEEWQSH IEGWEGSNIT DTCTEMLMCG VLLKISSGNI QERVFFLFDN LLVYCKRKHR RLKNSKASTD GHRYLFRGRI NTEVMEVENV DDGTADFHSS GHIVVNGWKI HNTAKNKWFV CMAKTPEEKH EWFEAILKER ERRKGLKLGM EQDTWVMISE QGEKLYKMMC RQGNLIKDRK RKLTTFPKCF LGSEFVSWLL EIGEIHRPEE GVHLGQALLE NGIIHHVTDK HQFKPEQMLY RFRYDDGTFY PRNEMQDVIS KGVRLYCRLH SLFTPVIRDK DYHLRTYKSV VMANKLIDWL IAQGDCRTRE EAMIFGVGLC DNGFMHHVLE KSEFKDEPLL FRFFSDEEME GSNMKHRLMK HDLKVVENVI AKSLLIKSNE GSYGFGLEDK NKVPIIKLVE KGSNAEMAGM EVGKKIFAIN GDLVFMRPFN EVDCFLKSCL NSRKPLRVLV STKPRETVKI PDSADGLGFQ IRGFGPSVVH AVGRGTVAAA AGLHPGQCII KVNGINVSKE THASVIAHVT ACRKYRRPTK QDSIQWVYNS IESAQEDLQK SHSKPPGDEA GDAFDCKVEE VIDKFNTMAI IDGKKEHVSL TVDNVHLEYG VVYEYDSTAG IKCNVVEKMI EPKGFFSLTA KILEALAKSD EHFVQNCTSL NSLNEVIPTD LQSKFSALCS ERIEHLCQRI SSYKKFSRVL KNRAWPTFKQ AKSKISPLHS SDFCPTNCHV NVMEVSYPKT STSLGSAFGV QLDSRKHNSH DKENKSSEQG KLSPMVYIQH TITTMAAPSG LSLGQQDGHG LRYLLKEEDL ETQDIYQKLL GKLQTALKEV EMCVCQIDDL LSSITYSPKL ERKTSEGIIP TDSDNEKGER NSKRVCFNVA GDEQEDSGHD TISNRDSYSD CNSNRNSIAS FTSICSSQCS SYFHSDEMDS GDELPLSVRI SHDKQDKIHS CLEHLFSQVD SITNLLKGQA VVRAFDQTKY LTPGRGLQEF QQEMEPKLSC PKRLRLHIKQ DPWNLPSSVR TLAQNIRKFV EEVKCRLLLA LLEYSDSETQ LRRDMVFCQT LVATVCAFSE QLMAALNQMF DNSKENEMET WEASRRWLDQ IANAGVLFHF QSLLSPNLTD EQAMLEDTLV ALFDLEKVSF YFKPSEEEPL VANVPLTYQA EGSRQALKVY FYIDSYHFEQ LPQRLKNGGG FKIHPVLFAQ ALESMEGYYY RDNVSVEEFQ AQINAASLEK VKQYNQKLRA FYLDKSNSPP NSTSKAAYVD KLMRPLNALD ELYRLVASFI RSKRTAACAN TACSASGVGL LSVSSELCNR LGACHIIMCS SGVHRCTLSV TLEQAIILAR SHGLPPRYIM QATDVMRKQG ARVQNTAKNL GVRDRTPQSA PRLYKLCEPP PPAGEETRTR PLEQKLISEE DLAANDILDY KDDDDKV
Purity :	> 80% as determined by SDS-PAGE and Coomassie blue staining
Stability :	Stable for 3 months from receipt of products under proper storage and handling conditions.
Storage :	Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
Concentration :	50 μg/mL as determined by BCA
Storage Buffer :	100 mM glycine, 25 mM Tris-HCl, pH 7.3.
SDS-PAGE :

Gene Name :	PREX2 phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2 [ Homo sapiens (human) ]
Official Symbol :	PREX2
Synonyms :	PREX2; phosphatidylinositol-3,4,5-trisphosphate dependent Rac exchange factor 2; DEP.2; DEPDC2; P-REX2; PPP1R129; phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 2 protein; DEP domain-containing protein 2; PtdIns(3,4,5)-dependent Rac exchanger 2; protein phosphatase 1, regulatory subunit 129
Gene ID :	80243
mRNA Refseq :	NM_024870
Protein Refseq :	NP_079146
MIM :	612139
UniProt ID :	Q70Z35