Recombinant Human PRF1 protein, His-SUMO-tagged

• Cat.No.: PRF1-26H
• Product Overview: Recombinant Human PRF1 protein(P14222)(22-555aa), fused with two N-terminal tags, His tag and SUMO tag, was expressed in E. coli.

Specification

• Source: E. coli
• Species: Human
• Tag: His-SUMO
• Form: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
  If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
• Molecular Mass: 75.2kDa
• Protein length: 22-555aa
• AA Sequence: PCHTAARSECKRSHKFVPGAWLAGE GVDVTSLRRSGSFPVDTQRFLRPDG TCTLCENALQEGTLQRLPLALTNWR AQGSGCQRHVTRAKVSSTEAVARDA ARSIRNDWKVGLDVTPKPTSNVHVS VAGSHSQAANFAAQKTHQDQYSFST DTVECRFYSFHVVHTPPLHPDFKRA LGDLPHHFNASTQPAYLRLISNYGT HFIRAVELGGRISALTALRTCELAL EGLTDNEVEDCLTVEAQVNIGIHGS ISAEAKACEEKKKKHKMTASFHQTY RERHSEVVGGHHTSINDLLFGIQAG PEQYSAWVNSLPGSPGLVDYTLEPL HVLLDSQDPRREALRRALSQYLTDR ARWRDCSRPCPPGRQKSPRDPCQCV CHGSAVTTQDCCPRQRGLAQLEVTF IQAWGLWGDWFTATDAYVKLFFGGQ ELRTSTVWDNNNPIWSVRLDFGDVL LATGGPLRLQVWDQDSGRDDDLLGT CDQAPKSGSHEVRCNLNHGHLKFRY HARCLPHLGGGTCLDYVPQMLLGEP PGNRSGAVW
• Purity: Greater than 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
• Storage: Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Gene Information

• Gene Name: PRF1 perforin 1 (pore forming protein) [ Homo sapiens ]
• Official Symbol: PRF1
• Synonyms: PRF1; perforin 1 (pore forming protein); perforin-1; HPLH2; P1; Perforin; perforin 1 (preforming protein); PFP; cytolysin; lymphocyte pore forming protein; lymphocyte pore-forming protein; FLH2; PFN1; MGC65093
• Gene ID: 5551
• mRNA Refseq: NM_001083116.1
• Protein Refseq: NP_001076585.1
• MIM: 170280
• UniProt ID: P14222