Active Recombinant Full Length Human Protein Kinase C, Iota, GST-tagged

• Cat.No.: PRKCI-405H
• Product Overview: Recombinant human full-length PKCiota was expressed by baculovirus inSf9 cellsusing an N-terminal GST tag. MW=98 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: GST
• Protein Length: 1-596 a.a.
• Description: PKCι is a member of the protein kinase C family of serine-threonine kinases. The amino acid sequence of PKCι showed greatest homology to PKCzeta, with 72% identity overall rising to 84% in the catalytic domain. Protein kinase C iota (PKCι) has been implicated in Ras signaling and is a critical downstream effector of oncogenic Ras in the colonic epithelium. Transgenic mice expressing constitutively active PKCι in the colon are highly susceptible to carcinogen-induced colon carcinogenesis.
• Sequence: Full-length.
• Applications: Kinase Assay, Western Blot.
• Storage And Stability: Store product at –70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Gene Information

• Gene Name: PRKCI protein kinase C, iota [ Homo sapiens ]
• Synonyms: PRKCI; protein kinase C, iota; PKCI; DXS1179E; MGC26534; nPKC-iota; EC 2.7.11.13; Protein kinase C iota type; Atypical protein kinase C-lambda/iota; aPKC-lambda/iota; PRKC-lambda/iota
• Gene ID: 5584
• mRNA Refseq: NM_002740
• Protein Refseq: NP_002731
• UniProt ID: P41743
• Chromosome Location: 3q26.3
• MIM: 600539
• Pathway: Tight junction; Signalling by NGF; Endocytosis; Insulin signaling pathway; Cell-cell adhesion systems
• Function: ATP binding; protein kinase C activity; metal ion binding; nucleotide binding; transferase activity; zinc ion binding; phospholipid binding; protein binding