Recombinant Human RAD50 293 Cell Lysate

• Cat.No.: RAD50-2557HCL

Specification

• Description: Antigen standard for RAD50 homolog (S. cerevisiae) (RAD50), transcript variant 1 is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
• Source: HEK 293 cells
• Species: Human
• Components: This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
• Size: 0.1 mg
• Storage Instruction: Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
• Applications: ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.

Gene Information

• Gene Name: RAD50 RAD50 homolog (S. cerevisiae) [ Homo sapiens ]
• Official Symbol: RAD50
• Synonyms: RAD50; RAD50 homolog (S. cerevisiae); RAD50 (S. cerevisiae) homolog; DNA repair protein RAD50; hRad50; RAD50 2; NBSLD; RAD502
• Gene ID: 10111
• mRNA Refseq: NM_005732
• Protein Refseq: NP_005723
• MIM: 604040
• UniProt ID: Q92878
• Chromosome Location: 5q23-q31
• Pathway: Assembly of the RAD50-MRE11-NBS1 complex at DNA double-strand breaks, organism-specific biosystem; BARD1 signaling events, organism-specific biosystem; BRCA1-associated genome surveillance complex (BASC), organism-specific biosystem; DNA Repair, organism-specific biosystem; Double-Strand Break Repair, organism-specific biosystem; Homologous Recombination Repair, organism-specific biosystem; Homologous recombination, organism-specific biosystem
• Function: contributes_to 3-5 exonuclease activity; ATP binding; contributes_to ATP-dependent DNA helicase activity; DNA binding; hydrolase activity; metal ion binding; nuclease activity; nucleotide binding; protein binding; protein binding, bridging; contributes_to single-stranded DNA specific endodeoxyribonuclease activity; zinc ion binding