Recombinant Human ROCK2 Protein, His-tagged

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Recombinant Human ROCK2 Protein, His-tagged

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Cat.No. : ROCK2-1879H
Product Overview : Recombinant human ROCK2 (5-554) was expressed using baculovirus in Sf9 insect cells with an N-terminal His tag.
Description : The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
Source : Insect cells
Species : Human
Tag : His
Bio-activity : 605 nmol/min/mg
Molecular Mass : ~66 kDa
Purity : >95%
Stability : One year at -70 centigrade from the date of shipment.
Storage : Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Concentration : 0.1 μg/μL
Storage Buffer : Recombinant protein stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25% glycerol.
Shipping : Shipped on dry ice.
Sample Kinase Activity Plot :

The specific of ROCK2 was determined be 605 nmol/min/mg

Purity :

The purity of ROCK2 was determined to be >95% by densitometry, approx. MW 66 kDa.

Gene Name : ROCK2 Rho associated coiled-coil containing protein kinase 2 [ Homo sapiens (human) ]
Official Symbol : ROCK2
Synonyms : ROCK2; Rho associated coiled-coil containing protein kinase 2; ROCK-II; p164 ROCK-2; rho-associated, coiled-coil-containing protein kinase II; EC 2.7.11.1; rho-associated protein kinase 2
Gene ID : 9475
mRNA Refseq : NM_004850
Protein Refseq : NP_004841
MIM : 604002
UniProt ID : O75116

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