Recombinant Human SIGLEC8 protein, His-tagged
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|Product Overview :||Recombinant Human SIGLEC8 (Met17-Ala363) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).|
|Description :||Siglec-8 is also known as SIGLEC8, SAF2, SIGLEC-8, SIGLEC8L and sialic acid binding Ig like lectin 8. Siglec-8 was first identified by CD33 homology screening of ESTs from a cDNA library generated from a patient diagnosed with idiopathic hypereosinophilic syndrome and was originally termed SAF-2 (sialoadhesin family 2). At the tissue level, Siglec-8 mRNA was found to be most highly expressed in lung, PBMCs, spleen, and kidney. Two splice variants of Siglec-8 exist. The initially characterized form contains 431 amino acid residues in total, subsequently, a longer form of Siglec-8, initially termed Siglec-8L. Both forms of Siglec-8 are found in eosinophils and contain a V-set domain with lectin activity and two C2-type Ig repeat domains in the extracellular region.|
|Predicted N Terminal :||Met17|
|Form :||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.|
|Molecular Mass :||The protein has a calculated MW of 39.7 kDa. The protein migrates as 50-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Protein length :||Met17-Ala363|
|Endotoxin :||Less than 1.0 EU per μg by the LAL method.|
|Purity :||>95% as determined by SDS-PAGE.|
For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Reconstitution :||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.|
|SDS-PAGE of SIGLEC8-772H :||
Human Siglec-8, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
|Gene Name :||SIGLEC8|
|Official Symbol :||SIGLEC8|
|Synonyms :||SIGLEC8; sialic acid binding Ig-like lectin 8; sialic acid-binding Ig-like lectin 8; MGC59785; SAF2; SIGLEC 8; SIGLEC8L; SAF-2; CDw329; sialoadhesin family member 2; SIGLEC-8|
|Gene ID :||27181|
|mRNA Refseq :||NM_014442|
|Protein Refseq :||NP_055257|
|UniProt ID :||Q9NYZ4|
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