Species : |
Human |
Source : |
E.coli |
Tag : |
Non |
Description : |
The γ-Synuclein (Originally known as a breast cancer specific gene product, BCSG1) is an acidic neuronal protein of 127 amino acids. The protein coding region of γ-synuclein was amplified by RT-PCR and cloned into an E.coli expression vector. |
Amino Acid Sequence : |
MDVFKKGFSI AKEGVVGAVE KTKQGVTEAA EKTKEGVMYV GAKTKENVVQ SVTSVAEKTK EQANAVSEAV VSSVNTVATK TVEEAENIAV TSGVVRKEDL RPSAPQQEGV ASKEKEEVAE EAQSGGD. |
Physical Appearance : |
Sterile filtered clorless solution. |
Formulation : |
The protein (1mg/ml) contains 20mM Tris-HCl buffer (pH 7.5), 0.1M NaCl and 1mM MgCl2. |
Purity : |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by reducing and non-reducing SDS-PAGE Coomassie. |
Stability : |
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
Protein Content : |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 1.4 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of BMP-6 as a Reference Standard. |
Storage : |
Lyophilized BMP 6 although stable at room temperature for 3 weeks, should be stored desiccated below-18℃. Upon reconstitution Bone Morphogenetic Protein 6 should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles. |