Recombinant Human SREBF1 Protein (1-490 aa), His-tagged

• Cat.No.: SREBF1-2715H
• Product Overview: Recombinant Human SREBF1 Protein (1-490 aa) is produced by in vitro E. coli expression system. This protein is fused with a 6xHis tag at the N-terminal. Research Area: Cardiovascular. Protein Description: Partial.

Specification

• Description: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway (By similarity). Binds to the sterol regulatory element 1 (SRE-1) (5'-ATCACCCCAC-3'). Has dual sequence specificity binding to both an E-box motif (5'-ATCACGTGA-3') and to SRE-1 (5'-ATCACCCCAC-3').
• Source: E. coli Cell Free
• Species: Human
• Tag: His
• Form: Tris-based buffer,50% glycerol
• Molecular Mass: 54.5 kDa
• Protein length: 1-490 aa
• AA Sequence: MDEPPFSEAALEQALGEPCDLDAAL LTDIEDMLQLINNQDSDFPGLFDPP YAGSGAGGTDPASPDTSSPGSLSPP PATLSSSLEAFLSGPQAAPSPLSPP QPAPTPLKMYPSMPAFSPGPGIKEE SVPLSILQTPTPQPLPGALLPQSFP APAPPQFSSTPVLGYPSPPGGFSTG SPPGNTQQPLPGLPLASPPGVPPVS LHTQVQSVVPQQLLTVTAAPTAAPV TTTVTSQIQQVPVLLQPHFIKADSL LLTAMKTDGATVKAAGLSPLVSGTT VQTGPLPTLVSGGTILATVPLVVDA EKLPINRLAAGSKAPASAQSRGEKR TAHNAIEKRYRSSINDKIIELKDLV VGTEAKLNKSAVLRKAIDYIRFLQH SNQKLKQENLSLRTAVHKSKSLKDL VSACGSGGNTDVLMEGVKTEVEDTL TPPPSDAGSPFQSSPLSLGSRGSGS GGSGSDSEPDSPVFEDSKAKPEQRP SLHSRGMLDRSRLAL
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA will be sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: SREBF1 sterol regulatory element binding transcription factor 1 [ Homo sapiens ]
• Official Symbol: SREBF1
• Synonyms: SREBF1; bHLHd1; SREBP 1c; SREBP1; SREBP-1; class D basic helix-loop-helix protein 1; SREBP-1c
• Gene ID: 6720
• mRNA Refseq: NM_001005291
• Protein Refseq: NP_001005291
• MIM: 184756
• UniProt ID: P36956