Recombinant Human STAT3 protein, His-tagged

Cat.No. : STAT3-31H
Product Overview : Recombinant Human STAT3 protein(Ile576~Pro678), fused with N-terminal His tag, was expressed in E. coli.
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Source : E. coli
Species : Human
Tag : His
Protein length : Ile576~Pro678
Form : 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose.
Bio-activity : Signal transducer and activator of transcription 3 (STAT3) is a member of the STAT protein family. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. STAT3 is phosphorylated by receptor-associated Janus kinases (JAK), form homo- or heterodimers, and translocate to the cell nucleus where they act as transcription activators. Besides, Epidermal Growth Factor Receptor 2 (EGFR2) has been identified as an interactor of STAT3, thus a binding ELISA assay was conducted to detect the interaction of recombinant human STAT3 and recombinant human EGFR2. Briefly, STAT3 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100μL were then transferred to EGFR2-coated microtiter wells and incubated for 2h at 37°C . Wells were washed with PBST and incubated for 1h with anti-STAT3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37°C. Finally, add 50μL stop solution to the wells and read at 450nm immediately. The binding activity of STAT3 and EGFR2wasshown in Figure, and this effect was in a dose dependent manner.
Molecular Mass : 12.9kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method.
Purity : >95%
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : STAT3 signal transducer and activator of transcription 3 (acute-phase response factor) [ Homo sapiens ]
Official Symbol : STAT3
Synonyms : STAT3; signal transducer and activator of transcription 3 (acute-phase response factor); signal transducer and activator of transcription 3; APRF; DNA-binding protein APRF; acute-phase response factor; HIES; FLJ20882; MGC16063;
Gene ID : 6774
mRNA Refseq : NM_003150
Protein Refseq : NP_003141
MIM : 102582
UniProt ID : P40763

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Customer Reviews (3)

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Reviews
11/17/2022

    Protein worked well in assay.

    04/21/2021

      Fast shipping, great customer service.

      02/06/2018

        Excellent purity, highly recommend.

        Q&As (7)

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        Are there any known inhibitors targeting STAT3 for therapeutic purposes? 02/01/2022

        Yes, there are several inhibitors targeting STAT3, including Stattic, S3I-201, and OPB-31121, which are being explored for their therapeutic potential in cancer and other diseases.

        Can STAT3 activation influence cell proliferation and survival? 11/16/2021

        Yes, activated STAT3 can promote cell proliferation and survival by inducing the expression of genes like cyclin D1 and Bcl-xL.

        How does STAT3 contribute to oncogenesis and tumor progression? 04/12/2021

        STAT3 can contribute to oncogenesis by promoting cell proliferation, survival, angiogenesis, and immune evasion, and by suppressing apoptosis in various cancer types.

        How can the activation of STAT3 be detected in cells? 02/24/2021

        Activation of STAT3 can be detected using Western blotting with phospho-specific antibodies that recognize the tyrosine-phosphorylated form of STAT3.

        How does STAT3 interact with other signaling pathways in the cell? 08/25/2020

        STAT3 can interact with other signaling pathways such as the MAPK, PI3K/AKT, and NF-κB pathways, leading to integrated cellular responses.

        What are the primary upstream activators of STAT3? 01/02/2019

        The primary upstream activators of STAT3 are cytokines and growth factors, especially those that signal through the Janus kinase (JAK) family, such as IL-6 and EGF.

        What role does STAT3 play in immune cell differentiation and function? 04/16/2018

        STAT3 plays a crucial role in the differentiation of Th17 cells, a subset of CD4+ T cells, and is also involved in the function of myeloid-derived suppressor cells (MDSCs) and regulatory T cells, influencing immune responses.

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