Recombinant Human Thrombopoietin

• Cat.No.: THPO-1533H
• Product Overview: Recombinant human rhTPO contains no μTagsμ and has a calculated MW of 37.4kDa. Asaresult of glycosylation, DTT-reduced protein migrates as a 60-80 kDa polypeptide in SDS-PAGE.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Description: Thrombopoietin (THPO) is a relatively lineage-specific cytokine that stimulates megakaryocyte growth and maturation in Thrombopoietin is a relatively lineage specific cytokine that stimulates megakaryocyte growth and maturation in vitro and is a potent in vivo thrombopoietic growth factor. On removal of the 21-amino acid signal peptide, the mature molecule consists of 2 domains: a receptor-binding domain that shows considerable homology to erythropoietin and a carbohydrate-rich carboxy-terminus of the protein that is highly glycosylated and important in maintaining protein stability.
• Form: Bulk protein in a 0.22 μm filtered solution of PBS, pH 7.4 and delivered as liquid formulation or lyophilized powder Normally 5-8% trehalose and lyophilized powder. Normally 5-8% trehalose and mannitol are added as protectants before lyophilization.
• Purity: >95% as determined by SDS-PAGE. All lots are greater than 95% pure.
• Endotoxin: Less than 1.0 EU per 1 μg of the rhTPO by the LAL method.
• Storage: Stable in lyophilized state at 4°C for 1 year after receipt. Sterile stock solutions reconstituted are stable at 4°C for 2 months and at -20°C for 6 months. Avoid repeated freeze-thaw cycles.

Gene Information

• Gene Name: THPO thrombopoietin [ Homo sapiens ]
• Official Symbol: THPO
• Synonyms: THPO; thrombopoietin; thrombopoietin nirs variant 1; megakaryocyte stimulating factor; MKCSF; c-mpl ligand; megakaryocyte colony-stimulating factor; MGDF; megakaryocyte growth and development factor; MPL ligand; myeloproliferative leukemia virus oncogene ligand; C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGC163194; Myeloproliferative leukemia virus oncogene ligand; ML; TPO; MPLLG; OTTHUMP00000210502; OTTHUMP00000210503; OTTHUMP00000210504
• Gene ID: 7066
• mRNA Refseq: NM_000460
• Protein Refseq: NP_000451
• MIM: 600044
• UniProt ID: P40225
• Chromosome Location: 3q27
• Pathway: Hematopoietic cell lineage; Formation of Platelet plug; Hemostasis; Platelet Activation; Platelet activation triggers
• Function: cytokine activity; growth factor activity; hormone activity