Recombinant Human Thyroid Stimulating Hormone Reference standard

• Cat.No.: TSH-198H
• Product Overview: This consists of a batch of ampoules containing recombinant DNA-derived human thyroid-stimulating hormone. The Reference Reagent for rDNA-derived TSH is intended for use in the validation of assay performance by the characterisation of systems which may distinguish between TSH from different sources.

Specification

• Species: Human
• Enzyme Unit: This preparation has been assigned a unitage of 6.7 milli Units ( or 0.0067 Units) per ampoule.
• Usage: For practical purposes each ampoule contains the same quantity of TSH. The entire contents of each ampoule should be completely dissolved in an accurately measured amount of buffer solution. No attempt should be made to weigh out portions of the freeze dried powder. The use of water to reconstitute ampoule contents is not recommended since results have indicated that under these conditions the recovery of material from the ampoule may be inconsistent with the assigned ampoule content. The material has not been sterilized and the ampoules contain no bacteriostat.
• Notes: This preparation is not for administration to humans. The preparation contains material of human origin, and either the final product or the source materials, from which it is derived, have been tested and found negative for HBsAg, anti-HIV and HCV RNA. As with all materials of biological origin, this preparation should be regarded as potentially hazardous to health. It should be used and discarded according to your own laboratorys safety procedures. Such safety procedures should include the wearing of protective gloves and avoiding the generation of aerosols. Care should be exercised in opening ampoules or vials, to avoid cuts.
• Storage: Unopened ampoules should be stored at -20°C. Please note: because of the inherent stability of lyophilized material, Creative Biomart may ship these materials at ambient temperature.
• Tag: Non

Reviews

07/11/2022

Good for Bimo-lecular fluorescence complementation control.

06/01/2020

TSH manufacturer's products are consistently of the highest quality, with a strong commitment to purity, potency, and consistency.

05/17/2020

Work great in Western blot analysis.

12/02/2019

The purity is truly exceptional, with no visible impurities or contaminants.

03/18/2019

TSH is ideal for Western-blot analysis, with strong bands and clear specificity.

04/09/2018

Can save materials and shorten the experiment time in luci-ferase complementation assay.

04/03/2018

It was accurately quantified in expression analysis.

03/11/2017

It is appropriate for T cell killing analysis.

Q&As

What the TSHR signal transduction, regulation, and maturation?

The TSHR, long known to signal via cAMP, can induce both the phospholipase C (PLC) and the protein kinase A signal transduction systems. Intracellular Ca2+ and PLC regulate iodide efflux, H2O2 production, and thyroglobulin iodination, while adenylate cyclase and cAMP regulate iodide uptake and transcription of thyroglobulin (Tg), thyroid peroxidase (TPO), and the sodium-iodide symporter.

Why the puzzling size diversity of TSHRs detected by western blotting?

Presumably by protein disulfide isomerase leading to release of the α subunit from the membrane-bound receptor.

What is associated with a high TSH value?

High-normal TSH-values are associated with an adverse lipid profile, high blood pressure, high body mass index (BMI), metabolic syndrome and fatal coronary heart disease.

How is TSH level regulated in brain?

It is known that the majority of the metabolically active T3 in the brain is not derived from the circulation, but produced locally by the effect of D2 on T4. Locally produced T3 largely acts on the hypothalamus and pituitary glands to control TSH levels.

Are TSH receptors expressed in places other than the thyroid gland?

Thyroid stimulating hormone receptors have been identified in mammalian heart, bone, kidneys, brain, skin, adipose, hair follicles, thymus, lymphocytes, astrocyte cultures and in fish testes.

How can thyroid cancer be treated by inhibiting thyrotropin?

Levothyroxine is recommended for long-term TSH suppression.

What cells or organs does TSH work on?

Including thyroid,lymphocytes, the pituitary, thymus, testes, kidney, brain, adipose tissue, and fibroblasts.

What is the biological function of thyroid stimulating hormone (TSH)?

The only established biologic function of thyroid stimulating hormone (TSH) is to regulate the synthesis and secretion of thyroid hormone from thyroid follicular cells.

How to achieve TSH transcription?

In response to low circulating thyroid hormone levels, the basal medial hypothalamus releases thyrotropin-releasing hormone (TRH) which then stimulates production of thyroid-stimulating hormone (TSH) from the thyrotropes of the anterior pituitary gland.

Besides TSH, what other substances can stimulate the release of prolactin?

Other substances like serotonin, gonadotropin-releasing hormone, and estrogen can also stimulate prolactin release.

What is the role of TSH protein in thyroid cancer?

In thyroid cancer, the TSH protein may have a role in promoting the proliferation and growth of cancer cells. Therefore, for some high-risk groups, such as patients with postoperative thyroidectomy, inhibition of TSH protein production and activity can reduce the risk of cancer recurrence.

What factors regulate the secretion of TSH protein?

The secretion of TSH protein is regulated by a variety of factors, including negative feedback regulation of the hypothalamic-pituitary-thyroid axis, thyroxine levels, and the regulatory mechanism of anterior pituitary cells.

Does the TSH protein have polymorphisms?

The polymorphisms of the TSH protein are partly related to the genetic variation of individuals. TSH proteins of different genotypes may affect an individual's sensitivity to TSH and regulation of thyroid function.

How does TSH protein regulate thyroid function?

TSH protein activates intracellular signaling pathways by binding to TSH receptors on the surface of thyroid cells, promoting thyroxine synthesis and secretion. It enhances the production of thyroxine by positively regulating iodine uptake, thyroglobulin synthesis, and thyroid peroxidase activity.

What are the applications of TSH protein in clinical diagnosis?

The TSH protein plays a key role in clinical diagnosis. By measuring serum TSH levels, it is possible to assess whether the thyroid gland is functioning normally for diagnosis and follow-up of thyroid-related diseases.

Does the TSH protein interact with other proteins?

Yes, the TSH protein can interact with TSH receptors on the surface of thyroid cells, triggering a series of signaling. In addition, TSH protein interacts with other cytokines and regulators to regulate thyroid function.