Recombinant Human TIMP Metallopeptidase Inhibitor 1

• Cat.No.: TIMP1-2886H
• Product Overview: Recombinant Human TIMP1 is expressed in Sf9 cells.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: Non
• Description: The tissue inhibitor of metalloproteinases (TIMP-1) is a glycoprotein with a molecular mass of about 28-kDa. The secreted protein consists of 184 amino acids, six disuilfide bonds and two glycosylation sites containing N-linked oligosaccharides. TIMP-1 specifically inhibits the extracellular-matrix-degrading metalloproteinases such as collagenase, gelatinase and stromelysin. It has no activity on mammalian membrane metallo-proteinases, procollagen peptidase, or on bacterial metalloproteinases such as thermolysin.
• Form: Liquid, in 50 mM Tris-HCl, pH 7; 200 mM NaCl; 5 mM CaCl2; 1 μM ZnCl2; 0.05% Brij 35; 0,05% NaN3.
• Specific Activity: It is recommended to perform a pre-incubation of about 20 min / 37°C which allows the formation of the enzyme-inhibitor complex.
• Purity: >92%, homogenous by electrophoresis and western blotting analysis.
• Stability And Storage: The TIMP-1 is very stable if stored at -70°C and can be kept at –20°C for several months and at 4°C for weeks without significant loss of activity. Repeated freezing and thawing should be avoided.

Gene Information

• Gene Name: TIMP1 TIMP metallopeptidase inhibitor 1 [ Homo sapiens ]
• Synonyms: TIMP1; TIMP metallopeptidase inhibitor 1; EPA; EPO; HCI; CLGI; TIMP; FLJ90373; tissue inhibitor of metalloproteinase 1; erythroid potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); Tissue inhibitor of metalloproteinases; TIMP-1; Collagenase inhibitor; OTTHUMP00000023214; Erythroid-potentiating activity
• Gene ID: 7076
• mRNA Refseq: NM_003254
• Protein Refseq: NP_003245
• MIM: 305370
• UniProt ID: P01033
• Chromosome Location: Xp11.3-p11.23
• Function: enzyme inhibitor activity; metalloendopeptidase inhibitor activity; protein binding