Active Recombinant Human TIMP1 protein

• Cat.No.: TIMP1-3194H
• Product Overview: Active Recombinant Human TIMP1 protein(NP_003245.1) (Cys 24-Ala 207) was expressed in HEK293.

Specification

• Species: Human
• Source: HEK293
• Tag: Non
• Protein Length: 24-207 aa
• Form: Lyophilized from sterile 20mM NaAC, 200mM NaCl, pH 5.5.
• Bio-activity: Measured by its ability to inhibit human MMP-2 cleavage of a fluorogenic peptide substrate MCA-PLGL-DPA-AR-NH2. The IC50 value is < 6 nM.
• Molecular Mass: The recombinant human TIMP1 comprises 184 amino acids with a predicted molecular mass of 21 kDa. It migrates as an apximately 26 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• protein Refseq-Weblink: http://www.ncbi.nlm.nih.gov/protein/NP_003245.1
• Unit ID: P01033
• Unit ID-Weblink: http://www.uniprot.org/uniprot/P01033

Gene Information

• Gene Name: TIMP1 TIMP metallopeptidase inhibitor 1 [ Homo sapiens ]
• Official Symbol: TIMP1
• Synonyms: TIMP1; TIMP metallopeptidase inhibitor 1; CLGI, TIMP, tissue inhibitor of metalloproteinase 1 (erythroid potentiating activity, collagenase inhibitor); metalloproteinase inhibitor 1; EPO; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1; EPA; HCI; CLGI; TIMP; FLJ90373
• Gene ID: 7076
• mRNA Refseq: NM_003254
• protein Refseq: NP_003245
• MIM: 305370