UGT1A9-100H
Optional Service: Optional requirements on this protein
Product Overview :
Recombinant human UGT1A9 protein without tag was expressed in insect cells.
Description :
This gene encodes a UDP-glucuronosyltransferase, an enzyme of the glucuronidation pathway that transforms small lipophilic molecules, such as steroids, bilirubin, hormones, and drugs, into water-soluble, excretable metabolites. This gene is part of a complex locus that encodes several UDP-glucuronosyltransferases. The locus includes thirteen unique alternate first exons followed by four common exons. Four of the alternate first exons are considered pseudogenes. Each of the remaining nine 5' exons may be spliced to the four common exons, resulting in nine proteins with different N-termini and identical C-termini. Each first exon encodes the substrate binding site, and is regulated by its own promoter. The enzyme encoded by this gene is active on phenols.
Source :
Insect cells
Species :
Human
Bio-activity :
UGT Activity Data(Unless otherwise stated, all assays are carried out at 37 centigrade in 100 mM Tris-HCl (pH 7.5) containing 5 mM magnesium chloride and 2 mM UDPGA. Alamethicin (10 μg/ml final concentration) is added to kinetics tests only) Substrate: 17β-estradiol (3-glucuronide formation) Linear with time up to: 12 min(Determined at 4 μM substrate, using a protein concentration of 25 μg/ml) Linear with protein up to: 20 μg/ml(Determined at 4 μM substrate, using an incubation time of 5 min) Vmax: 10832 pmol/min/mg protein Km: 2.3 μM
Usage :
For laboratory (research) purposes only
Storage :
Store at -80 centigrade. Avoid frequent temperature changes. Thaw on ice.
Concentration :
Protein Concentration: 5 mg/ml
Storage Buffer :
25 mM Tris-acetate (pH 7.6), 10% (v/v) glycerol, 1 mM EDTA
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.