Species : |
Human |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
Met1-Tyr380 |
Tag : |
C-His |
Form : |
Lyophilized from sterile PBS, 8% Glycerol, 5% trehalose, pH 7.4. |
Bio-activity : |
Label Recombinant Human Netrin G1 / NTNG1 Protein, His Tag with biotin. Loaded Biotinylated Recombinant Human Netrin G1 / NTNG1 Protein on SA Biosensor, can bind Recombinant Human UNC5C Protein, His Tag with an affinity constant of 1.28 μM as determined in BLI assay (Sartorius Octet RED384). |
Molecular Mass : |
The recombinant Human UNC5C consisting of 351 amino acids and has a calculated molecular mass of 39.99 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 48.7 kDa protein in SDS-PAGE under reducing conditions. |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
≥ 90 % as determined by SDS-PAGE. ≥ 90 % as determined by SEC-HPLC. |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |