Recombinant Human USP14 Protein, Myc/DDK-tagged, C13 and N15-labeled

• Cat.No.: USP14-4165H
• Product Overview: USP14 MS Standard C13 and N15-labeled recombinant protein (NP_005142) with a C-terminal MYC/DDK tag, was expressed in HEK293 cells.

Specification

• Description: This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
• Source: HEK293
• Species: Human
• Tag: Myc&DDK
• Molecular Mass: 56.1 kDa
• AA Sequence: MPLYSVTVKWGKEKFEGVELNTDEP PMVFKAQLFALTGVQPARQKVMVKG GTLKDDDWGNIKIKNGMTLLMMGSA DALPEEPSAKTVFVEDMTEEQLASA MELPCGLTNLGNTCYMNATVQCIRS VPELKDALKRYAGALRASGEMASAQ YITAALRDLFDSMDKTSSSIPPIIL LQFLHMAFPQFAEKGEQGQYLQQDA NECWIQMMRVLQQKLEAIEDDSVKE TDSSSASAATPSKKKSLIDQFFGVE FETTMKCTESEEEEVTKGKENQLQL SCFINQEVKYLFTGLKLRLQEEITK QSPTLQRNALYIKSSKISRLPAYLT IQMVRFFYKEKESVNAKVLKDVKFP LMLDMYELCTPELQEKMVSFRSKFK DLEDKKVNQQPNTSDKKSSPQKEVK YEPFSFADDIGSNNCGYYDLQAVLT HQGRSSSSGHYVSWVKRKQDEWIKF DDDKVSIVTPEDILRLSGGGDWHIA YVLLYGPRRVEIMEEESEQTRTRPL EQKLISEEDLAANDILDYKDDDDKV
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 3 months from receipt of products under proper storage and handling conditions.
• Storage: Store at -80 centigrade. Avoid repeated freeze-thaw cycles.
• Concentration: 50 μg/mL as determined by BCA
• Storage Buffer: 100 mM glycine, 25 mM Tris-HCl, pH 7.3.

Gene Information

• Gene Name: USP14 ubiquitin specific peptidase 14 [ Homo sapiens (human) ]
• Official Symbol: USP14
• Synonyms: USP14; ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase); ubiquitin specific protease 14 (tRNA guanine transglycosylase); ubiquitin carboxyl-terminal hydrolase 14; TGT; ubiquitin thioesterase 14; deubiquitinating enzyme 14; ubiquitin thiolesterase 14; ubiquitin-specific processing protease 14; ubiquitin-specific-processing protease 14; tRNA-guanine transglycosylase, 60-kD subunit; ubiquitin specific protease 14 (tRNA-guanine transglycosylase)
• Gene ID: 9097
• mRNA Refseq: NM_005151
• Protein Refseq: NP_005142
• MIM: 607274
• UniProt ID: P54578