Recombinant Human vacuolar protein sorting 4 homolog B (S. cerevisiae), His-tagged VPS4B-269H

Recombinant Human vacuolar protein sorting 4 homolog B (S. cerevisiae), His-tagged

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Cat.No. : VPS4B-269H Optional Service: Optional requirements on this protein
Product Overview : VPS4B, 1-444aa, Human, His tag, E.coli
Description : Vacuolar protein sorting-associated protein 4B, also known as VPS4B, belongs to the AAA (ATPases associated with diverse cellular activities) family. It is involved in late steps of the endosomal multivesicular bodies (MVB) pathway and recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Dominant negative mutant of VPS4B inhibit vacuolar protein sorting and also arrest HIV-1 and MLV budding. Recombinant human VPS4B protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Source : E.coli
Species : Human
Tag : His-tag
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT
Molecular Mass : 51.8 kDa (468aa)
AA Sequence : MGSSHHHHHH SSGLVPRGSH MGSHMSSTSP NLQKAIDLAS KAAQEDKAGN YEEALQLYQH AVQYFLHVVK YEAQGDKAKQ SIRAKCTEYL DRAEKLKEYL KNKEKKAQKP VKEGQPSPAD EKGNDSDGEG ESDDPEKKKL QNQLQGAIVI ERPNVKWSDV AGLEGAKEAL KEAVILPIKF PHLFTGKRTP WRGILLFGPP GTGKSYLAKA VATEANNSTF FSISSSDLVS KWLGESEKLV KNLFQLAREN KPSIIFIDEI DSLCGSRSEN ESEAARRIKT EFLVQMQGVG VDNDGILVLG ATNIPWVLDS AIRRRFEKRI YIPLPEPHAR AAMFKLHLGT TQNSLTEADF RELGRKTDGY SGADISIIVR DALMQPVRKV QSATHFKKVR GPSRADPNHL VDDLLTPCSP GDPGAIEMTW MDVPGDKLLE PVVSMSDMLR SLSNTKPTVN EHDLLKLKKF TEDFGQEG
Purity : >90% by SDS - PAGE
Storage : Can be stored at +4C short term (1-2 weeks). For long term storage, aliquot and store at -20C or -70C. Avoid repeated freezing and thawing cycles.
Concentration : 0.25mg/ml (determined by Bradford assay)
Gene Name : VPS4B vacuolar protein sorting 4 homolog B (S. cerevisiae) [ Homo sapiens ]
Official Symbol : VPS4B
Synonyms : VPS4B; vacuolar protein sorting 4 homolog B (S. cerevisiae); SKD1, suppressor of K+ transport defect 1 , vacuolar protein sorting 4B (yeast); vacuolar protein sorting-associated protein 4B; SKD1B; VPS4 2; cell migration-inducing 1; vacuolar protein sorting 4B; suppressor of K+ transport defect 1; cell migration-inducing gene 1 protein; suppressor of K(+) transport growth defect 1; MIG1; SKD1; VPS4-2;
Gene ID : 9525
mRNA Refseq : NM_004869
Protein Refseq : NP_004860
MIM : 609983
UniProt ID : O75351
Chromosome Location : 18q21.33
Pathway : CXCR4-mediated signaling events, organism-specific biosystem; Endocytosis, organism-specific biosystem; Endocytosis, conserved biosystem; Endosomal Sorting Complex Required For Transport (ESCRT), organism-specific biosystem; Membrane Trafficking, organism-specific biosystem;
Function : ATP binding; ATPase activity; ATPase activity; ATPase activity, coupled; nucleotide binding; protein C-terminus binding; protein binding;
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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