Active Recombinant Human VCAM1 protein, His tagged

• Cat.No.: VCAM1-3142H
• Product Overview: Active Recombinant Human VCAM1 protein(Phe 25-Pro 697)(NP_001069.1) was expressed in Human Cells, fused with a polyhistidine tag at the N-terminus.

Specification

• Species: Human
• Source: Human Cells
• Tag: His
• Protein Length: 25-697 aa
• Form: Lyophilized from sterile PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to VCAM1 coated plates (40 μg/mL, 100 μL/well) approximately 30%-70% will adhere for 1 hour incubation at 37°C.
• Molecular Mass: The recombinant human VCAM1 consists of 689 amino acids and predicts a molecular mass of 76 kDa. As a result of glycosylation, rhVCAM1 migrates as an approximately 110 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Store it under sterile conditions at -20°C to -80°C upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: VCAM1 vascular cell adhesion molecule 1 [ Homo sapiens ]
• Official Symbol: VCAM1
• Synonyms: VCAM1; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; CD106; CD106 antigen; INCAM-100; MGC99561; DKFZp779G2333
• Gene ID: 7412
• mRNA Refseq: NM_001078
• Protein Refseq: NP_001069
• MIM: 192225
• UniProt ID: P19320