"VEGFA" Related Products

Recombinant Human VEGF165 Protein (Ala 27 - Arg 191), His-tagged

Cat.No. : VEGFA-382H
Product Overview : Recombinant Human VEGF165 Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4 ). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.1 kDa. The protein migrates as 24 kDa and 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source : HEK293
Species : Human
Tag : His
Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity : Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human VEGF165 Protein, His-tagged with a linear range of 0.2-4 ng/mL (QC tested).
Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Recombinant Human VEGF165 Protein, His-tagged with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Molecular Mass : 21.1 kDa
Protein length : Ala 27 - Arg 191
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >90% as determined by SDS-PAGE.
Notes : Please avoid repeated freeze-thaw cycles.
Stability : No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Figure :

Gene Name : VEGFA vascular endothelial growth factor A [ Homo sapiens ]
Official Symbol : VEGFA
Synonyms : VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; VEGF; MVCD1; MGC70609;
Gene ID : 7422
mRNA Refseq : NM_001025366
Protein Refseq : NP_001020537
UniProt ID : P15692

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.