"VEGFA" Related Products

Recombinant Human VEGF165 Protein (Ala 27 - Arg 191), His-tagged

Cat.No.: VEGFA-382H
Product Overview: Recombinant Human VEGF165 Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4 ). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.1 kDa. The protein migrates as 24 kDa and 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Source: HEK293
Species: Human
Tag : His
Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Bio-activity: Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human VEGF165 Protein, His-tagged with a linear range of 0.2-4 ng/mL (QC tested).
Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Recombinant Human VEGF165 Protein, His-tagged with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Molecular Mass: 21.1 kDa
Protein length: Ala 27 - Arg 191
Endotoxin: Less than 1.0 EU per μg by the LAL method.
Purity: >90% as determined by SDS-PAGE.
Notes: Please avoid repeated freeze-thaw cycles.
Stability: No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
Gene Name: VEGFA vascular endothelial growth factor A [ Homo sapiens ]
Official Symbol: VEGFA
Synonyms: VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; VEGF; MVCD1; MGC70609;
Gene ID: 7422
mRNA Refseq: NM_001025366
Protein Refseq: NP_001020537
UniProt ID: P15692

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.