|Product Overview:||Recombinant Human VEGF165 Protein, His-tagged is expressed from human 293 cells (HEK293). It contains AA Ala 27 - Arg 191 (Accession # P15692-4 ). Predicted N-terminus: His. This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 21.1 kDa. The protein migrates as 24 kDa and 25-27 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.|
|Form:||Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.|
|Bio-activity:||Immobilized Human VEGF R1&VEGF R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Recombinant Human VEGF165 Protein, His-tagged with a linear range of 0.2-4 ng/mL (QC tested).
Avastin (Bevacizumab) captured on CM5 chip via anti-human IgG Fc antibodies surface, can bind Recombinant Human VEGF165 Protein, His-tagged with an affinity constant of 1.03 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
|Molecular Mass:||21.1 kDa|
|Protein length:||Ala 27 - Arg 191|
|Endotoxin:||Less than 1.0 EU per μg by the LAL method.|
|Purity:||>90% as determined by SDS-PAGE.|
|Notes:||Please avoid repeated freeze-thaw cycles.|
|Stability:||No activity loss is observed after storage at:
4-8 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
|Storage:||For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.|
|Reconstitution:||It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 mg/ml. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.|
|Gene Name:||VEGFA vascular endothelial growth factor A [ Homo sapiens ]|
|Synonyms:||VEGFA; vascular endothelial growth factor A; vascular endothelial growth factor , VEGF; VEGF A; VPF; VEGF; MVCD1; MGC70609;|