Recombinant Human ZAP70 protein, His & T7-tagged

• Cat.No.: ZAP70-1490H
• Product Overview: Recombinant Human ZAP70 aa. (Leu337~Leu600 (Accession # P43403)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: This gene encodes an enzyme belonging to the protein tyrosine kinase family, and it plays a role in T-cell development and lymphocyte activation. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T-cells. Two transcript variants that encode different isoforms have been found for this gene.
• Source: E. coli
• Species: Human
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.2kDa
• Protein length: Leu337~Leu600 (Accession # P43403)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Isoelectric Point: 8.6

Gene Information

• Gene Name: ZAP70 zeta chain of T-cell receptor associated protein kinase 70 [ Homo sapiens (human) ]
• Official Symbol: ZAP70
• Synonyms: ZAP70; zeta chain of T-cell receptor associated protein kinase 70; SRK; STD; TZK; STCD; IMD48; ADMIO2; ZAP-70; tyrosine-protein kinase ZAP-70; 70 kDa zeta-associated protein; 70 kDa zeta-chain associated protein; syk-related tyrosine kinase; zeta chain of T cell receptor associated protein kinase 70kDa; zeta-chain (TCR) associated protein kinase 70kDa; zeta-chain associated protein kinase, 70kD
• Gene ID: 7535
• mRNA Refseq: NM_001079.3
• Protein Refseq: NP_001070.2
• UniProt ID: P43403