Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, Fc-tagged (HPLC-verified)

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Recombinant Human/Cynomolgus/Rhesus macaque CD28 protein, Fc-tagged (HPLC-verified)

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Cat.No. : CD28-562H
Product Overview : Recombinant Human/Cynomolgus/Rhesus macaque CD28 (Asn19-Pro152) protein (HPLC-verified) protein was fused to human IgG1 Fc tag at C-terminus and expressed in human 293 cells (HEK293).
Description : T-cell-specific surface glycoprotein CD28 is also known as TP44, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. is one of the molecules expressed on T cells that provide co-stimulatory signals, which are required for T cell activation. CD28 is the receptor for CD80 (B7.1) and CD86 (B7.2). When activated by Toll-like receptor ligands, the CD80 expression is upregulated in antigen presenting cells (APCs). The CD86 expression on antigen presenting cells is constitutive. CD28 is the only B7 receptor constitutively expressed on naive T cells.
Source : HEK293
Species : Human/Cynomolgus/Rhesus macaque
Tag : human IgG1 Fc
Predicted N Terminal : Asn19
Form : Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5, 10% trehalose.
Molecular Mass : The protein has a calculated MW of 41.6 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Protein length : Asn19-Pro152
Endotoxin : Less than 1.0 EU per μg by the LAL method.
Purity : >95% as determined by SDS-PAGE.
Storage : For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20 to -70 centigrade for 12 months in lyophilized state;
-70 centigrade for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
SDS-PAGE of CD28-562H :

Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

SEC-HPLC of CD28-562H :

The purity of Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) was greater than 90% as determined by SEC-HPLC.

Bioactivity-ELISA :

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-2, Fc Tag with a linear range of 0.019-0.625 μg/mL (QC tested).

Bioactivity-ELISA of CD28-562H :

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) at 2 μg/mL (100 μL/well) can bind Anti-Mouse CD28 MAb with a linear range of 0.1-2 ng/ml (QC tested).

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) at 5 μg/mL (100 μL/well) can bind Biotinylated Human B7-1, Fc Tag with a linear range of 0.313-5 μg/mL (Routinely tested).

Bioactivity-SPR of CD28-562H :

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, His Tag with an affinity constant of 5.29 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) (Cat. No. CD8-H525a) on CM5 Chip can bind Human B7-1, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. B71-H52A4) with an affinity constant of 2.72 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Immobilized Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on CM5 Chip can bind Human B7-1, Fc Tag (HPLC-verified) with an affinity constant of 2.01 μM as determined in a SPR assay (Biacore T200) (Routinely tested).

Bioactivity-BLI of CD28-562H :

Loaded Human / Cynomolgus / Rhesus macaque CD28, Fc Tag (HPLC-verified) on ProteinA Biosensor, can bind Human B7-1, His Tag with an affinity constant of 2.2 μM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

Gene Name : CD28
Official Symbol : CD28
Synonyms : Tp44
Gene ID : 940
mRNA Refseq : NM_006139.4
Protein Refseq : NP_006130.1
UniProt ID : P10747

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