Recombinant Monkey EGFR Protein, His-tagged, Alexa Fluor 647 conjugated

• Cat.No.: EGFR-61CAF647
• Product Overview: Alexa Fluor 647 conjugated recombinant Rhesus macaque EGFR (Leu25-Ser645) protein was fused to His-tag at C-terminus and expressed in human 293 cells (HEK293).

Specification

• Species: Monkey
• Source: HEK293
• Tag: His
• Protein Length: Leu25-Ser645
• Description: The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer.
• Form: Lyophilized
• Molecular Mass: The protein has a calculated MW of 70.5 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• N-terminal Sequence Analysis: Leu 25
• Endotoxin: < 1.0 EU/ μg by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE
• Characteristic: Disulfide-linked homodimer
  Labeled with Alexa Fluor 647 via amines
  Excitation = 650 nm
  Emission = 668 nm
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% trehalose.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.
• Conjugation: Alexa Fluor 647

Gene Information

• Gene Name: EGFR
• Official Symbol: EGFR
• Gene ID: 613027
• mRNA Refseq: XM_015133436
• Protein Refseq: XP_014988922
• UniProt ID: P55245